The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development.
The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development.
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油菜多聚半乳糖醛酸酶基因 BcMF2 与内脏发育相关
DOI：
10.1093/jxb/ern295
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发表时间：
2009
影响因子：
6.9
通讯作者：
Liu T
中科院分区：
文献类型：
--
作者：
Huang L;Cao J;Zhang A;Ye Y;Zhang Y;Liu T
Brassica Campestris Male Fertility 2 (BcMF2) 是一种假定的多聚半乳糖醛酸酶 (PG) 基因，之前从大白菜 (Brassica Campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis) 的花蕾中分离出来。发现该基因在花药发育四分体阶段后的绒毡层和花粉中特异性表达。采用反义RNA技术研究BcMF2在大白菜中的功能。扫描和透射电镜显示转基因成熟花粉粒存在畸形，如生发沟位置异常。此外，面向外部的均匀果胶外内层似乎过度发育并主要占据内内，从而颠倒了内内内层和外外内层的正常比例分布。由于它是内壁的延续，花粉管壁不能正常生长。这导致近80%的转基因花粉粒的花粉管尖端形成气球状膨胀结构。在这些转基因植物中还发现绒毡层过早降解，显示 BcMF2 基因表达降低。因此，BcMF2 可能编码一种新的 PG，其在花粉壁发育中发挥重要作用，可能是通过调节果胶的动态代谢来实现的。
Brassica campestris Male Fertility 2 (BcMF2) is a putative polygalacturonase (PG) gene previously isolated from the flower bud of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis). This gene was found to be expressed specifically in tapetum and pollen after the tetrad stage of anther development. Antisense RNA technology was used to study the function of BcMF2 in Chinese cabbage. Scanning and transmission electron microscopy revealed that there were deformities in the transgenic mature pollen grains such as abnormal location of germinal furrows. In addition, the homogeneous pectic exintine layer facing the exterior seemed to be overdeveloped and predominantly occupied the intine, thus reversing the normal proportional distribution of the internal endintine layer and the external exintine layer. Since it is a continuation of the intine layer, the pollen tube wall could not grow normally. This resulted in the formation of a balloon-like swelling structure in the pollen tube tip in nearly 80% of the transgenic pollen grains. Premature degradation of tapetum was also found in these transgenic plants, which displayed decreased expression of the BcMF2 gene. BcMF2 might therefore encode a new PG with an important role in pollen wall development, possibly via regulation of pectin's dynamic metabolism.
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影响因子：
11.6
作者：
BROWN, SM;CROUCH, ML
通讯作者：
CROUCH, ML
影响因子：
7.4
作者：
Pina, C;Pinto, F;Becker, JD
通讯作者：
Becker, JD
DOI：
10.1093/protein/14.9.615
发表时间：
2001-09-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
Markovic, O;Janecek, S
通讯作者：
Janecek, S
影响因子：
2.8
作者：
Li, Yanyan;Cao, Jiashu;Xiang, Xun
通讯作者：
Xiang, Xun
影响因子：
2.9
作者：
PaxsonSowders, DM;Owen, HA;Makaroff, CA
通讯作者：
Makaroff, CA