Effect of norharmane in vitro on juvenile hormone epoxide hydrolase activity in the lower termite, Reticulitermes speratus.
Effect of norharmane in vitro on juvenile hormone epoxide hydrolase activity in the lower termite, Reticulitermes speratus.
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DOI：
10.1673/031.008.1301
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Enoki A
中科院分区：
文献类型：
--
作者：
Itakura S;Kawabata S;Tanaka H;Enoki A
采用气相色谱/质谱联用技术，测定了散白蚁（Reticulitermes speratus（Kolbe））工蚁、蚁和拟蚁体内芳香族β-咔啉去甲哈尔烷的含量。5月份收集的工蚁、若虫和拟蚁体内的降哈曼含量（0.4.3 ng/白蚁）高于11月份的含量（0.0.2 ng/白蚁）。在整个动物和脂肪体的组织切片中观察到去甲哈尔烷的荧光。去甲哈尔烷，在1 mM的最终浓度，刺激保幼激素环氧化物水解酶（JHEH）活性的酶提取物从麦角类，但抑制JHEH活性在较高的浓度。去甲骆驼蓬烷刺激的JHEH活性升高可能会促进R. speratus。
The aromatic β-carboline norharmane was determined in workers, nymphs, and ergatoids of Reticulitermes speratus (Kolbe) (Isoptera: Rhinotermitidae) by gas chromatography/mass spectrometry. Norharmane levels in workers, nymphs, and ergatoids collected in May (∼4.3 ng/termite) were higher than those in November (∼0.2 ng/termite). Fluorescence of norharmane was observed in histological sections in whole animals and in the fat body. Norharmane, at a final concentration of 1 mM, stimulated juvenile hormone epoxide hydrolase (JHEH) activity of enzyme extract from ergatoids, but inhibited JHEH activity at higher concentrations. The elevated JHEH activity stimulated by norharmane should accelerate juvenile hormone metabolism in R. speratus.
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通讯作者：
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