Comparative effect of chlormadinone acetate and diethylstilbestrol as promoters in mammary tumorigenesis of rats irradiated with γ-rays during lactation
Comparative effect of chlormadinone acetate and diethylstilbestrol as promoters in mammary tumorigenesis of rats irradiated with γ-rays during lactation
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醋酸氯地孕酮和己烯雌酚作为促进剂对哺乳期γ射线照射大鼠乳腺肿瘤发生的影响比较
DOI：
10.1023/a:1006149608475
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发表时间：
2004
影响因子：
3.8
通讯作者：
K. Wakabayashi
中科院分区：
文献类型：
--
作者：
H. Inano;Keiko Suzuki;M. Onoda;Hisae Kobayashi;K. Wakabayashi
本研究的目的是确定雌激素和黄体酮在放射性乳腺肿瘤发展中的促进作用。为消除卵巢内源性激素对肿瘤的促进作用，所有大鼠在哺乳期第21天接受2.6 Gy γ射线照射后立即切除卵巢，分为3组。对照实验，照射1个月后给大鼠植入胆固醇颗粒。在1年的植入期间，只有1只大鼠发生了纤维腺瘤（4.3%的乳腺肿瘤发病率）。另外两组分别给予醋酸氯麦地那酮（CMA）提高黄体酮水平或己烯雌酚（DES）提高雌激素活性作为肿瘤促进剂，疗程1年。与对照组相比，CMA治疗并没有显著增加乳腺肿瘤的发生率。然而，使用DES导致乳腺肿瘤发生率明显高于对照组（79.3%）。与胆固醇治疗相比，DES治疗引起血清催乳素浓度升高（5倍），雌二醇-17β浓度降低（对照组的22%）。这些结果表明，DES是辐射引发的乳腺细胞肿瘤发生的有效促进剂，而CMA则不是。DES可能通过与内质网结合直接作用于照射后的乳腺细胞，也可能通过刺激垂体分泌催乳素间接作用于照射后的乳腺细胞。
The purpose of this study was to determine the promotional role of estrogen and progestin in the development of radiation-induced mammary tumors. To eliminate the effects of endogenous ovarian hormones on tumor promotion, all rats were ovariectomized immediately after the initiation by irradiation with 2.6 Gy γ-rays at day 21 of lactation, and were divided into 3 groups. For the control experiment, rats were implanted with a cholesterol pellet 1 month after the irradiation. Only one rat developed a fibroadenoma (4.3% mammary tumor incidence) during the 1 year period of the implantation. In the other two groups, chlormadinone acetate (CMA) to increase progestin level or diethylstilbestrol (DES) to increase estrogenic activity were administered, respectively, as tumor promoters for 1 year. Treatment with CMA did not significantly increase the incidence of mammary tumors as compared with the controls. However, administration of DES resulted in a significantly higher mammary tumor incidence (79.3%) than control treatment. Compared with cholesterol administration, DES treatment caused an increase in prolactin concentration in serum (5-fold), and reduction of estradiol-17β concentration (22% of control). These results suggest that DES ia a potent effective promoter for tumorigenesis of radiation-initiated mammary cells, but CMA is not. DES may act directly on the irradiated mammary cells by binding to ER, and indirectly by stimulating prolactin secretion from the pituitary glands.
DOI：
10.1007/978-1-4612-0485-5_15
发表时间：
1990-03
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
J. Russo;B. Gusterson;A. E. Rogers;I. Russo;S. Wellings;M. V. van Zwieten
通讯作者：
J. Russo;B. Gusterson;A. E. Rogers;I. Russo;S. Wellings;M. V. van Zwieten
DOI：
10.1016/s0021-9258(18)34893-2
发表时间：
1982-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Maurer
通讯作者：
R. Maurer