Purification and Characterization of the Heat‐Stable Factors Essential for the Conversion of Lignoceric Acid to Cerebronic Acid and Glutamic Acid: Identification of N‐Acetyl‐l‐Aspartic Acid
Purification and Characterization of the Heat‐Stable Factors Essential for the Conversion of Lignoceric Acid to Cerebronic Acid and Glutamic Acid: Identification of N‐Acetyl‐l‐Aspartic Acid
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木蜡酸转化为脑酸和谷氨酸所必需的热稳定性因子的纯化和表征：N-乙酰基-L-天冬氨酸的鉴定
DOI：
10.1111/j.1471-4159.1983.tb08052.x
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发表时间：
1983
影响因子：
4.7
通讯作者：
C. Fenselau
中科院分区：
文献类型：
--
作者：
H. Shigematsu;N. Okamura;H. Shimeno;Y. Kishimoto;L. Kan;C. Fenselau
Abstract: The conversion of lignoceric acid to cerebronic acid, ceramides, cerebrosides, and glutamic acid is catalyzed by a rat brain particulate preparation. The heat‐stable factor, prepared from calf cerebellum, together with the heat‐labile factor, a pyridine nucleotide, and Mg2+ are essential to all of these metabolic pathways. Our previous work showed that the heat‐stable factor is composed of at least two components, HSF‐1 and HSF‐2, and identified HSF‐2 as d‐glucose‐6‐phosphate. In the current investigation, HSF‐1 was further purified and found to be N‐acetyl‐l‐aspartic acid. In addition, it was discovered that a third component, HSF‐3, is also required for heat‐stable factor activity. A reconstituted system composed of N‐acetylaspartic acid, glucose‐6‐phosphate, and HSF‐3 fully replaced the heat‐stable factor essential for the conversion of lignoceric acid to cerebronic acid and glutamic acid. The reconstituted heat‐stable factor did not show the initial time lag always observed with the crude heat‐stable factor.
在吡啶核苷酸和胞质因子存在下，通过大鼠大脑制备，由二十四烷酸形成谷氨酸：极长链脂肪酸的大脑特异性氧化。
影响因子：
2.9
作者：
Uda,M;Singh,I;Kishimoto,Y
通讯作者：
Kishimoto,Y