Male-killer symbiont screening reveals novel associations in Adalia ladybirds
Male-killer symbiont screening reveals novel associations in Adalia ladybirds
复制标题
雄性杀手共生体筛选揭示了阿达利亚瓢虫中的新关联
DOI:
10.1099/acmi.0.000585.v1
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发表时间:
2023
期刊:
影响因子:
--
通讯作者:
Archer J
中科院分区:
文献类型:
--
作者:
Archer J
While male-killing bacteria are known to infect across arthropods, ladybird beetles represent a hotspot for these symbioses. In some host species, there are multiple different symbionts that vary in presence and frequency between populations. To further our understanding of spatial and frequency variation, we tested for the presence of three male-killing bacteria:Wolbachia,RickettsiaandSpiroplasma, in twoAdalialadybird species from a previously unexplored UK population. The two-spot ladybird,A. bipunctata, is known to harbour all three male-killers, and we identifiedSpiroplasmainfection in the Merseyside population for the first time. However, in contrast to previous studies on two-spot ladybirds from continental Europe, evidence from egg-hatch rates indicates theSpiroplasmastrain present in the Merseyside population does not cause embryonic male-killing. In the related ten-spot ladybird,A. decempunctata, there is only one previous record of a male-killing symbiont, aRickettsia, which we did not detect in the Merseyside sample. However, PCR assays indicated the presence of aSpiroplasmain a singleA. decempunctataspecimen. Marker sequence indicated that thisSpiroplasmawas divergent from that found in sympatricA. bipunctata. Genome sequencing of theSpiroplasma-infectedA. decempunctataadditionally revealed the presence of cobionts in the form of aCentistesparasitoid wasp and the parasitic fungiBeauveria. Further study ofA. decempunctatafrom this population is needed to resolve whether it is the ladybird or wasp cobiont that harboursSpiroplasma, and to establish the phenotype of this strain. These data indicate first that microbial symbiont phenotype should not be assumed from past studies conducted in different locations, and second that cobiont presence may confound screening studies aimed to detect the frequency of a symbiont in field collected material from a focal host species.
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影响因子:
5.8
作者:
Pollmann, Marie;Moore, Logan D.;Krimmer, Elena;D'Alvise, Paul;Hasselmann, Martin;Perlman, Steve J.;Ballinger, Matthew J.;Steidle, Johannes L. M.;Gottlieb, Yuval
通讯作者:
Gottlieb, Yuval
影响因子:
5.1
作者:
Sharon Towett;J. Morrow;Shannon Close;J. Royer;M. Riegler
通讯作者:
M. Riegler
DOI:
10.1007/bf02769875
发表时间:
1997-01-01
期刊:
ENTOMOPHAGA
影响因子:
--
作者:
Majerus, MEN;Hurst, GDD
通讯作者:
Hurst, GDD
DOI:
--
发表时间:
--
期刊:
--
影响因子:
--
作者:
Systems Biology
通讯作者:
Systems Biology
影响因子:
7.2
作者:
Hurst, Gregory D. D.;Frost, Crystal L.
通讯作者:
Frost, Crystal L.