Male-killer symbiont screening reveals novel associations in Adalia ladybirds

Male-killer symbiont screening reveals novel associations in Adalia ladybirds
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雄性杀手共生体筛选揭示了阿达利亚瓢虫中的新关联

DOI:
10.1099/acmi.0.000585.v1
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发表时间:
2023
期刊:
--
影响因子:
--
通讯作者:
Archer J
Archer J
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--
作者:
Archer J

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虽然已知杀死雄性的细菌会在节肢动物中感染,但瓢虫是这些共生的热点。在一些寄主物种中,存在多种不同的共生体,它们的存在和频率在不同种群之间有所不同。为了进一步了解空间和频率变化,我们测试了三种杀死雄性的细菌:沃尔巴克氏杆菌、立克次体和螺旋体,在来自以前未被探索的英国种群的两种Adalialadybird中。众所周知,双斑瓢虫携带着所有三种雄性杀手,我们首次在默西塞德郡的种群中发现了螺旋体感染。然而,与之前对欧洲大陆两点瓢虫的研究不同,来自卵孵化率的证据表明,默西塞德种群中存在的螺旋体菌株不会导致胚胎雄性死亡。在相关的十点瓢虫A.decempunctata中,只有一种先前记录的杀死雄性的共生体aRickettsia,我们在默西塞德的样本中没有检测到。然而,聚合酶链式反应分析表明存在螺旋体a SingleA。这是一项重大的任务。标记序列表明,该螺旋体与在sympatricA中发现的有所不同。这是一份点心。螺旋体感染者的基因组测序。此外,还发现了以蜈蚣寄生蜂和寄生真菌白僵菌的形式存在的共生体。进一步研究OFA。为了确定滋生螺旋体的是瓢虫还是黄蜂,并确定该菌株的表型,需要从该种群中分离出细菌。这些数据表明,首先,不应根据过去在不同地点进行的研究假设微生物共生体的表型,其次,共生体的存在可能会混淆旨在检测从局部寄主物种采集的野外材料中共生体频率的筛选研究。
While male-killing bacteria are known to infect across arthropods, ladybird beetles represent a hotspot for these symbioses. In some host species, there are multiple different symbionts that vary in presence and frequency between populations. To further our understanding of spatial and frequency variation, we tested for the presence of three male-killing bacteria:Wolbachia,RickettsiaandSpiroplasma, in twoAdalialadybird species from a previously unexplored UK population. The two-spot ladybird,A. bipunctata, is known to harbour all three male-killers, and we identifiedSpiroplasmainfection in the Merseyside population for the first time. However, in contrast to previous studies on two-spot ladybirds from continental Europe, evidence from egg-hatch rates indicates theSpiroplasmastrain present in the Merseyside population does not cause embryonic male-killing. In the related ten-spot ladybird,A. decempunctata, there is only one previous record of a male-killing symbiont, aRickettsia, which we did not detect in the Merseyside sample. However, PCR assays indicated the presence of aSpiroplasmain a singleA. decempunctataspecimen. Marker sequence indicated that thisSpiroplasmawas divergent from that found in sympatricA. bipunctata. Genome sequencing of theSpiroplasma-infectedA. decempunctataadditionally revealed the presence of cobionts in the form of aCentistesparasitoid wasp and the parasitic fungiBeauveria. Further study ofA. decempunctatafrom this population is needed to resolve whether it is the ladybird or wasp cobiont that harboursSpiroplasma, and to establish the phenotype of this strain. These data indicate first that microbial symbiont phenotype should not be assumed from past studies conducted in different locations, and second that cobiont presence may confound screening studies aimed to detect the frequency of a symbiont in field collected material from a focal host species.
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DOI: --
发表时间: 2021
影响因子: 5.1
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