Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe.
Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe.
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DOI：
10.1016/j.neuron.2012.06.040
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发表时间：
2012-09-06
期刊：
影响因子：
16.2
通讯作者：
Pieribone VA
中科院分区：
文献类型：
--
作者：
Jin L;Han Z;Platisa J;Wooltorton JR;Cohen LB;Pieribone VA
使用基于荧光蛋白的电压传感器监测神经元的电活动一直受到现有探针响应幅度小和动力学速度慢的限制。在这里，我们报道了一种名为ArcLight的新型荧光蛋白电压传感器的开发，以及随着电压变化而表现出荧光强度大幅变化的衍生探针。ArcLight由肠道黄瓜电压敏感磷酸酶的电压敏感结构域和携带点突变A227D的超黄道PHluorin组成。当在HEK 293细胞中测量到+100 mV去极化时，ArcLight A242的荧光强度降低了35%，这是先前报道的荧光蛋白电压传感器信号的五倍多。我们发现，这些新探针的信号大小和响应速度的结合，首次允许在单个神经元和树突中可靠地检测单个动作电位和兴奋电位。
Monitoring neuronal electrical activity using fluorescent protein-based voltage sensors has been limited by small response magnitudes and slow kinetics of existing probes. Here we report the development of a novel fluorescent protein voltage sensor, named ArcLight, and derivative probes that exhibit large changes in fluorescence intensity in response to voltage changes. ArcLight consists of the voltage-sensing domain of Ciona intestinalis voltage-sensitive phosphatase and super ecliptic pHluorin that carries the point mutation A227D. The fluorescence intensity of ArcLight A242 decreases 35% in response to +100mV depolarization when measured in HEK 293 cells, which is more than five times larger than the signals from previously reported fluorescent protein voltage sensors. We show that the combination of signal size and response speed of these new probes, for the first time, allows the reliable detection of single action potentials and excitatory potentials in individual neurons and dendrites.
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影响因子：
3.4
作者：
Agmon, N
通讯作者：
Agmon, N
影响因子：
3.7
作者：
Dimitrov D;He Y;Mutoh H;Baker BJ;Cohen L;Akemann W;Knöpfel T
通讯作者：
Knöpfel T
影响因子：
3
作者：
Baker, B. J.;Lee, H.;Kosmidis, E. K.
通讯作者：
Kosmidis, E. K.
影响因子：
48
作者：
Tsutsui, Hidekazu;Karasawa, Satoshi;Miyawaki, Atsushi
通讯作者：
Miyawaki, Atsushi
影响因子：
2.9
作者：
Elsliger, MA;Wachter, RM;Remington, SJ
通讯作者：
Remington, SJ