Exercise ameliorates chronic kidney disease-induced defects in muscle protein metabolism and progenitor cell function.
Exercise ameliorates chronic kidney disease-induced defects in muscle protein metabolism and progenitor cell function.
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DOI：
10.1038/ki.2009.260
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发表时间：
2009-10
影响因子：
19.6
通讯作者：
Mitch, William E.
中科院分区：
文献类型：
--
作者：
Wang, Xiaonan H.;Du, Jie;Klein, Janet D.;Bailey, James L.;Mitch, William E.
慢性肾病（CKD）损害肌肉蛋白质代谢，导致肌肉萎缩，运动可以抵消这种肌肉消耗。在这里，我们评估了阻力运动（肌肉超负荷）和耐力训练（跑步机运行）如何影响CKD诱导的肌肉蛋白质代谢和祖细胞功能异常，使用小鼠跖肌。两种运动模型都减弱了疾病诱导的肌肉蛋白水解的增加，并改善了Akt和叉头转录因子FoxO1的磷酸化。肌肉超负荷，但不是跑步机运行，纠正蛋白质合成和蛋白质合成介质的水平，如磷酸化的mTOR和p70S6K在CKD小鼠的肌肉。在这些小鼠中，肌肉超负荷，但不是跑步机，跑步，增加肌肉祖细胞的数量和活动，测量的MyoD，肌细胞生成素，和eMyHC mRNA的量。肌肉超负荷不仅增加了CKD小鼠的跖重量，减少了肌肉蛋白水解，而且纠正了调节蛋白质和祖细胞功能的细胞内信号。跑步机跑步纠正肌肉蛋白水解，但不纠正蛋白质合成或祖细胞功能。我们的研究结果为评估不同类型的运动对慢性肾脏病患者肌肉萎缩的影响提供了依据。
Chronic kidney disease (CKD) impairs muscle protein metabolism leading to muscle atrophy, and exercise can counteract this muscle wasting. Here we evaluated how resistance exercise (muscle overload) and endurance training (treadmill running) affect CKD-induced abnormalities in muscle protein metabolism and progenitor cell function using mouse plantaris muscle. Both exercise models blunted the increase in disease-induced muscle proteolysis and improved phosphorylation of Akt and the forkhead transcription factor FoxO1. Muscle overloading, but not treadmill running, corrected protein synthesis and levels of mediators of protein synthesis such as phosphorylated mTOR and p70S6K in the muscles of mice with CKD. In these mice, muscle overload, but not treadmill, running, increased muscle progenitor cell number and activity as measured by the amounts of MyoD, myogenin, and eMyHC mRNAs. Muscle overload not only increased plantaris weight and reduced muscle proteolysis but also corrected intracellular signals regulating protein and progenitor cell function in mice with CKD. Treadmill running corrects muscle proteolysis but not protein synthesis or progenitor cell function. Our results provide a basis for evaluating different types of exercise on muscle atrophy in patients with chronic kidney disease.
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