Damnacanthal, a noni component, exhibits antitumorigenic activity in human colorectal cancer cells.
Damnacanthal, a noni component, exhibits antitumorigenic activity in human colorectal cancer cells.
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DOI：
10.1016/j.jnutbio.2011.04.017
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发表时间：
2012-08
影响因子：
5.6
通讯作者：
Baek, Seung Joon
中科院分区：
文献类型：
--
作者：
Nualsanit, Thararat;Rojanapanthu, Pleumchitt;Gritsanapan, Wandee;Lee, Seong-Ho;Lawson, Darunee;Baek, Seung Joon
虎刺醛是从巴戟天（Morinda citrifolia L.）（诺丽），其已用于包括癌症在内的多种慢性疾病的传统治疗。虽然诺丽在亚洲和波利尼西亚国家已经消费了很长一段时间，但它发挥多种益处的分子机制开始出现。在这份报告中，我们研究了系统的方法对癌抑制能力的damnacanthal在结直肠肿瘤的发生。虎刺醛在结肠直肠癌细胞中表现出细胞生长停滞以及半胱天冬酶活性诱导。我们还检测了几种潜在的靶蛋白，发现促凋亡蛋白非甾体抗炎激活基因-1（NAG-1）被高度诱导。随后，我们发现，damnacanthal也增强转录因子C/EBPβ，它控制NAG-1的转录活性。在存在damnacanthal的情况下，通过shRNA阻断C/EBPβ导致NAG-1表达降低以及caspase活性降低。综上所述，这些结果表明，damnacanthal增加人结直肠癌细胞中的抗肿瘤活性，并且C/EBPβ在damnacanthal诱导的NAG-1表达中起作用。
Damnacanthal, an anthraquinone compound, is isolated from the roots of Morinda citrifolia L. (noni), which has been used for traditional therapy in several chronic diseases including cancer. Although noni has been consumed for a long time in Asian and Polynesian countries, the molecular mechanisms by which it exerts several benefits are starting to emerge. In this report, we examined systematic approaches on the cancer suppressing capability of damnacanthal in colorectal tumorigenesis. Damnacanthal exhibits cell growth arrest as well as caspase activity induction in colorectal cancer cells. We also examined several potential target proteins and found that the pro-apoptotic protein Nonsteroidal anti-inflammatory activated gene-1 (NAG-1) is highly induced. Subsequently, we have found that damnacanthal also enhances transcription factor C/EBPβ, which controls NAG-1 transcriptional activity. Blocking of C/EBPβ by shRNA results in the reduction of NAG-1 expression as well as caspase activity in the presence of damnacanthal. Taken together, these results indicate that damnacanthal increases anti-tumorigenic activity in human colorectal cancer cells, and C/EBPβ plays a role in damnacanthal-induced NAG-1 expression.
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影响因子：
3.5
作者：
Hiwasa, T;Arase, Y;Suzuki, N
通讯作者：
Suzuki, N
影响因子：
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