Immuno-physiological adaptations confer wax moth Galleria mellonella resistance to Bacillus thuringiensis.
Immuno-physiological adaptations confer wax moth Galleria mellonella resistance to Bacillus thuringiensis.
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DOI：
10.1080/21505594.2016.1164367
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发表时间：
2016-11-16
期刊：
影响因子：
5.2
通讯作者：
Butt TM
中科院分区：
文献类型：
--
作者：
Dubovskiy IM;Grizanova EV;Whitten MM;Mukherjee K;Greig C;Alikina T;Kabilov M;Vilcinskas A;Glupov VV;Butt TM
微进化机制的细菌病原体的抗性进行了探讨，在人口的大蜡螟，大蜡螟，选择了8.8倍增加对昆虫病原细菌苏云金芽孢杆菌（Bt）的抗性相比，非选择（可疑）线。比较了抗性和易感昆虫品系的防御策略，以揭示抗性的基础机制，以及这些生存策略的可能成本。在未感染的状态下，抗性昆虫表现出增强的基础基因表达的再生和改善Bt毒素活性的中肠。此外，这些昆虫还表现出与脂肪体中的炎症/压力管理和免疫防御相关的基因活性升高。经口感染Bt后，这些基因的表达在两个品系的脂肪体和中肠中进一步升高，并且在更大程度上，其中一些基因在抗性品系中的表达比敏感品系中的表达更高。该基因表达分析揭示了针对Bt损伤位点的抗性机制模式，昆虫更加重视组织修复，如脂肪体和中肠上皮中这些基因的表达升高所揭示的。与易感昆虫不同，Bt感染显著降低了抗性昆虫肠道微生物群的多样性和丰富度（丰度）。这些观察结果表明，抗性品系不仅具有更完整的中肠，而且还将抗微生物因子分泌到肠腔中，这不仅减轻了Bt活性，而且还影响了其他肠道细菌的活力。值得注意的是，抗性品系采用多因素适应对Bt的抗性，而没有任何检测到的负面权衡，因为昆虫表现出更高的繁殖力。
Microevolutionary mechanisms of resistance to a bacterial pathogen were explored in a population of the Greater wax moth, Galleria mellonella, selected for an 8.8-fold increased resistance against the entomopathogenic bacterium Bacillus thuringiensis (Bt) compared with a non-selected (suspectible) line. Defense strategies of the resistant and susceptible insect lines were compared to uncover mechanisms underpinning resistance, and the possible cost of those survival strategies. In the uninfected state, resistant insects exhibited enhanced basal expression of genes related to regeneration and amelioration of Bt toxin activity in the midgut. In addition, these insects also exhibited elevated activity of genes linked to inflammation/stress management and immune defense in the fat body. Following oral infection with Bt, the expression of these genes was further elevated in the fat body and midgut of both lines and to a greater extent some of them in resistant line than the susceptible line. This gene expression analysis reveals a pattern of resistance mechanisms targeted to sites damaged by Bt with the insect placing greater emphasis on tissue repair as revealed by elevated expression of these genes in both the fat body and midgut epithelium. Unlike the susceptible insects, Bt infection significantly reduced the diversity and richness (abundance) of the gut microbiota in the resistant insects. These observations suggest that the resistant line not only has a more intact midgut but is secreting antimicrobial factors into the gut lumen which not only mitigate Bt activity but also affects the viability of other gut bacteria. Remarkably the resistant line employs multifactorial adaptations for resistance to Bt without any detected negative trade off since the insects exhibited higher fecundity.
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影响因子：
3.4
作者：
Grizanova, E. V.;Dubovskiy, I. M.;Glupov, V. V.
通讯作者：
Glupov, V. V.
影响因子：
4.4
作者：
Gunning, RV;Dang, HT;Moores, GD
通讯作者：
Moores, GD
影响因子：
1.4
作者：
Martínez-Ramírez, AC;Gould, F;Ferré, J
通讯作者：
Ferré, J
CLOVR：使用云计算从桌面上自动化和便携式序列分析的虚拟机。
影响因子：
3
作者：
Angiuoli SV;Matalka M;Gussman A;Galens K;Vangala M;Riley DR;Arze C;White JR;White O;Fricke WF
通讯作者：
Fricke WF
DOI：
10.1073/pnas.0604865103
发表时间：
2006-10-10
影响因子：
11.1
作者：
Broderick, Nichole A.;Raffa, Kenneth F.;Handelsman, Jo
通讯作者：
Handelsman, Jo