Cloning and expression analysis of mevalonate kinase and phosphomevalonate kinase genes associated with the MVA pathway in Santalum album.
Cloning and expression analysis of mevalonate kinase and phosphomevalonate kinase genes associated with the MVA pathway in Santalum album.
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檀香MVA通路相关甲羟戊酸激酶和磷酸甲羟戊酸激酶基因的克隆及表达分析
DOI：
10.1038/s41598-021-96511-4
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发表时间：
2021-08-19
影响因子：
4.6
通讯作者：
Ma G
中科院分区：
文献类型：
--
作者：
Niu M;Xiong Y;Yan H;Zhang X;Li Y;da Silva JAT;Ma G
檀香（Santalum album L.）因其芳香的心材和提取的油而受到高度重视。桑塔洛尔是这种油的主要成分，是萜类化合物，它们通过甲羟戊酸（MVA）途径生物合成。甲valonate kinase （MK）和phosphomevalonate kinase （PMK）是MVA通路中的关键酶。目前对S. album中编码MK和PMK的基因以及调控其表达的机制知之甚少。为了分离和鉴定参他洛尔合成的功能基因，从参他洛尔中克隆了一个MK基因（SaMK）和一个PMK基因（SaPMK）。分析了这些基因的序列。通过生物信息学分析，鉴定了SaMK和SaPMK基因与其他植物的MK和PMK基因的同源性。我们还研究了SaMK和SaPMK蛋白的亚细胞定位，以及SaMK和SaPMK在酵母中的功能互补。结果表明，SaMK和SaPMK的cDNA全长分别为1409 bp和1679 bp。SaMK包含一个1381 bp的开放阅读框（ORF），编码460个氨基酸的多肽，SaPMK包含一个1527 bp的开放阅读框（ORF），编码508个氨基酸的多肽。SaMK和SaPMK与其他植物的MK和PMK基因具有较高的同源性。SaMK在MK缺陷突变株YMR208W中的功能互补和SaPMK在PMK缺陷突变株YMR220W中的功能互补证实，克隆的SaMK和SaPMK cDNA分别编码功能性MK和PMK，介导酵母中MVA的生物合成。组织表达模式分析显示，SaMK和SaPMK在所有测试组织中均组成性表达。SaMK在幼叶中高表达，在边材中表达较弱。SaPMK在根和成熟叶中高表达，在幼叶中低表达。几种诱导子的诱导实验表明，茉莉酸甲酯上调了SaMK和SaPMK的表达。这些结果将有助于进一步研究MK和PMK基因在桑他洛尔合成过程中的作用。
Sandalwood (Santalum album L.) is highly valued for its fragrant heartwood and extracted oil. Santalols, which are the main components of that oil, are terpenoids, and these are biosynthesized via the mevalonic acid (MVA) pathway. Mevalonate kinase (MK) and phosphomevalonate kinase (PMK) are key enzymes in the MVA pathway. Little is known about the genes that encode MK and PMK in S. album or the mechanism that regulates their expression. To isolate and identify the functional genes involved in santalol biosynthesis in S. album, an MK gene designated as SaMK, and a PMK gene designated as SaPMK, were cloned from S. album. The sequences of these genes were analyzed. A bioinformatics analysis was conducted to assess the homology of SaMK and SaPMK with MK and PMK genes from other plants. The subcellular localization of SaMK and SaPMK proteins was also investigated, as was the functional complementation of SaMK and SaPMK in yeast. Our results show that the full-length cDNA sequences of SaMK and SaPMK were 1409 bp and 1679 bp long, respectively. SaMK contained a 1381 bp open reading frame (ORF) encoding a polypeptide of 460 amino acids and SaPMK contained a 1527 bp ORF encoding a polypeptide of 508 amino acids. SaMK and SaPMK showed high homology with MK and PMK genes of other plant species. Functional complementation of SaMK in a MK-deficient mutant yeast strain YMR208W and SaPMK in a PMK-deficient mutant yeast strain YMR220W confirmed that cloned SaMK and SaPMK cDNA encode a functional MK and PMK, respectively, mediating MVA biosynthesis in yeast. An analysis of tissue expression patterns revealed that SaMK and SaPMK were constitutively expressed in all the tested tissues. SaMK was highly expressed in young leaves but weakly expressed in sapwood. SaPMK was highly expressed in roots and mature leaves, but weakly expressed in young leaves. Induction experiments with several elicitors showed that SaMK and SaPMK expression was upregulated by methyl jasmonate. These results will help to further study the role of MK and PMK genes during santalol biosynthesis in S. album.
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DOI：
10.1126/science.1191652
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ajikumar PK;Xiao WH;Tyo KE;Wang Y;Simeon F;Leonard E;Mucha O;Phon TH;Pfeifer B;Stephanopoulos G
通讯作者：
Stephanopoulos G
DOI：
10.1007/s10295-009-0592-1
发表时间：
2009-08-01
影响因子：
3.4
作者：
Albers, Eva;Larsson, Christer
通讯作者：
Larsson, Christer
影响因子：
5.2
作者：
Gao W;Hillwig ML;Huang L;Cui G;Wang X;Kong J;Yang B;Peters RJ
通讯作者：
Peters RJ
影响因子：
15
作者：
COREY, EJ;YAMAMOTO, H
通讯作者：
YAMAMOTO, H
银杏萜三内酯生物合成中乙酰辅酶A C-乙酰转移酶和甲羟戊酸激酶基因的分子克隆、表征和功能分析
DOI：
10.3390/molecules22010074
发表时间：
2017-01-02
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Chen Q;Yan J;Meng X;Xu F;Zhang W;Liao Y;Qu J
通讯作者：
Qu J