Analysis of endogenous glutathione-adducts and their metabolites.
Analysis of endogenous glutathione-adducts and their metabolites.
复制标题
DOI：
10.1002/bmc.1374
复制
发表时间：
2010-01
影响因子：
1.8
通讯作者：
Blair, Ian A.
中科院分区：
文献类型：
--
作者：
Blair, Ian A.
对谷胱甘肽 (GSH) 加合物及其代谢物进行验证分析的能力对于确定它们是否在细胞生化或病理生理过程中发挥作用至关重要。稳定同位素稀释 (SID) 方法与液相色谱-串联质谱 (LC-MS/MS) 的结合使用为此类分析提供了最高的生物分析特异性。定量研究通常需要通过使用多反应监测 (MRM)/MS 获得的高灵敏度，而不是灵敏度低得多但更具体的全扫描方法。该方法采用对应于完整分子的母离子以及通过碰撞诱导解离获得的显着产物离子。使用 SID LC-MRM/MS，分析物必须与验证过程中建立的重同位素内标、正确的母离子和正确的子离子具有相同的相对 LC 保留时间。用于生物标志物分析的任何其他生物分析技术都无法达到这种水平的特异性。本综述将描述 SID LC-MR/MS 方法在 GSH 加合物及其代谢物分析中的应用。它还将讨论使用这种方法来严格确定其作为疾病和暴露生物标志物的效用的潜在未来方向。
The ability to conduct validated analyses of glutathione (GSH)-adducts and their metabolites is critically important in order to establish whether they play a role in cellular biochemical or pathophysiological processes. The use of stable isotope dilution (SID) methodology in combination with liquid chromatography–tandem mass spectrometry (LC-MS/MS) provides the highest bioanalytical specificity possible for such analyses. Quantitative studies normally require the high sensitivity that can be obtained by the use of multiple reaction monitoring (MRM)/MS rather than the much less sensitive but more specific full scanning methodology. The method employs a parent ion corresponding to the intact molecule together with a prominent product ion that obtained by collision induced dissociation. Using SID LC-MRM/MS, analytes must have the same relative LC retention time to the heavy isotope internal standard established during the validation procedure, the correct parent ion and the correct product ion. This level of specificity cannot be attained with any other bioanalytical technique employed for biomarker analysis. This review will describe the application of SID LC-MR/MS methodology for the analysis of GSH-adducts and their metabolites. It will also discuss potential future directions for the use of this methodology for rigorous determination of their utility as disease and exposure biomarkers.
登录
查看更多内容
影响因子：
4.1
作者：
Chandrasena, R. Esala P.;Edirisinghe, Praneeth D.;Thatcher, Gregory R. J.
通讯作者：
Thatcher, Gregory R. J.
DOI：
10.1016/j.jchromb.2009.08.011
发表时间：
2009-10-01
影响因子：
3
作者：
Armstrong, Michael;Liu, Andrew H.;Reisdorph, Nichole
通讯作者：
Reisdorph, Nichole
影响因子：
3.9
作者：
BERGHOLTE, JM;SOBERMAN, RJ;OKITA, RT
通讯作者：
OKITA, RT
影响因子：
4.1
作者：
Bolton, JL;Trush, MA;Monks, TJ
通讯作者：
Monks, TJ
影响因子：
4.8
作者：
Burczynski, ME;Sridhar, GR;Penning, TM
通讯作者：
Penning, TM