Regional myocardial perfusion under exchange transfusion with liposomal hemoglobin: in vivo and in vitro studies using rat hearts.
Regional myocardial perfusion under exchange transfusion with liposomal hemoglobin: in vivo and in vitro studies using rat hearts.
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脂质体血红蛋白换血下的局部心肌灌注：使用大鼠心脏的体内和体外研究。
DOI：
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
F. Kajiya
中科院分区：
文献类型：
--
作者：
T. Matsumoto;T. Asano;K. Mano;H. Tachibana;M. Todoh;M. Tanaka;F. Kajiya
本研究的目的是检验脂质体血红蛋白 (LH) 换血可减少局部心肌血流的微观异质性同时维持心脏功能的假设。使用与白蛋白混合的新红细胞作为LH溶液，其中LH体积分数为17约18%，血红蛋白密度比大鼠血液小近三分之二。在有或没有50%血液-LH换血的麻醉大鼠中，通过示踪数字射线照相术（100μm分辨率）测量左心室游离壁的局部心肌流量。 LH 输注 (n = 8) 的层内流量分布表现出比没有 (n = 8) LH 输注更低的异质性。 3,3-二氨基联苯胺染色证实没有血红蛋白外渗（n = 2）。由于 LH 输注，颈动脉流量增加了 68%，而动脉压和心率保持不变。另一方面，使用交叉循环的大鼠心脏 (n = 7) 来评估 300 次/分钟起搏和 100 mmHg 灌注压下 50% 血液-LH 交换对冠脉流量和张力保持的影响。血流中断 30 秒后，血液-LH 交换导致充血期间冠脉流量增加 71%，流量增加百分比下降 10%。心肌O(2) 供应和消耗分别增加9% 和10%，而心肌O(2) 提取量保持不变。 LH 共灌注引起的体内颈动脉流量和交叉循环心脏中冠状动脉流量的大幅增加可以通过表观流量粘度的降低来解释。这些结果表明，在 LH 共灌注下，心肌血流的微观不均匀性随着冠状动脉血流的增加而降低，同时相当程度地保持冠状动脉张力和心脏功能。
The purpose of this study was to test the hypothesis that exchange transfusion with liposomal hemoglobin (LH) reduces the microheterogeneity of regional myocardial flows while sustaining cardiac function. Neo Red Cell mixed with albumin was used as the LH solution, in which the LH volume fraction was 17 approximately 18% and hemoglobin density was nearly two-thirds smaller than in rat blood. Regional myocardial flows in left ventricular free walls were measured by tracer digitalradiography (100-mum resolution) in anesthetized rats with or without 50% blood-LH exchange transfusion. Within-layer flow distributions showed lower heterogeneity with (n = 8) than without (n = 8) LH transfusion. No extravasation of hemoglobin was confirmed by 3,3-diaminobenzidin staining (n = 2). Carotid flow increased by 68% due to LH transfusion, whereas arterial pressure and heart rate remained unchanged. On the other hand, cross-circulated rat hearts (n = 7) were used to evaluate the effects of 50% blood-LH exchange on coronary flow and tone preservation under 300-beats/min pacing and 100-mmHg perfusion pressure. Blood-LH exchange caused a 71% increase of coronary flow and 10% decrease of percent flow increase during hyperemia after 30-s flow interruption. Myocardial O(2) supply and consumption increased by 9% and 10%, respectively, whereas myocardial O(2) extraction remained unchanged. The large increases of in vivo carotid flow and coronary flow in cross-circulated hearts due to LH coperfusion could be explained by the reduction of apparent flow viscosity. These results suggest that under LH coperfusion, the microheterogeneity of myocardial flows decreases with increased coronary flow while fairly preserving coronary tone and cardiac function.
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影响因子：
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作者：
DESJARDINS, C;DULING, BR
通讯作者：
DULING, BR
影响因子：
20.1
作者：
BASSINGTHWAIGHTE, JB;MALONE, MA;KROHN, KA
通讯作者：
KROHN, KA
Hb 囊泡对失血性休克和复苏的全身和微血管反应。
DOI：
10.1152/ajpheart.00080.2002
发表时间：
2002
期刊：
American journal of physiology. Heart and circulatory physiology
影响因子：
--
作者：
Sakai,Hiromi;Takeoka,Shinji;Wettstein,Reto;Tsai,AmyG;Intaglietta,Marcos;Tsuchida,Eishun
通讯作者：
Tsuchida,Eishun
影响因子：
--
作者：
KASSAB, GS;FUNG, YCB
通讯作者：
FUNG, YCB
心肌血流和代谢的异质性：异常兴奋加剧。
DOI：
10.1016/s0002-9149(99)00250-7
发表时间：
1999
期刊：
The American journal of cardiology
影响因子：
--
作者：
Bassingthwaighte,JB;Li,Z
通讯作者：
Li,Z