Presence of anti-Müllerian hormone (AMH) during follicular development in the porcine ovary.
Presence of anti-Müllerian hormone (AMH) during follicular development in the porcine ovary.
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猪卵巢卵泡发育过程中存在抗苗勒管激素 (AMH)。
DOI：
10.1371/journal.pone.0197894
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Teerds KJ
中科院分区：
文献类型：
--
作者：
Almeida FRCL;Costermans NGJ;Soede NM;Bunschoten A;Keijer J;Kemp B;Teerds KJ
抗苗勒管激素（AMH）由发育中卵泡的颗粒细胞表达，并通过确定卵泡刺激素阈值水平在卵泡募集的周期过程中起抑制作用。了解AMH在猪卵巢中的表达对于了解母猪的繁殖效率具有重要意义。在本研究中，我们研究了AMH的表达在卵泡发育的青春期前和成年母猪免疫组织化学，激光捕获显微切割和RT-qPCR。尽管猪卵巢AMH的免疫组织化学定位在许多方面与其他物种没有差异，但也存在一些显著差异。与大多数物种一样，AMH在猪卵泡发育期间首次出现在原始卵泡的梭形颗粒细胞中，并继续存在于颗粒细胞中直至窦期。当卵泡达到排卵前阶段时，AMH染色强度显著增加，蛋白质和基因表达不仅限于颗粒细胞;卵泡膜细胞现在也表达AMH。AMH在排卵后继续在黄体的黄体细胞中表达，这是猪卵巢特有的现象。AMH在黄体中的生理功能目前尚不清楚。可以推测，它可能有助于调节小窦卵泡的周期性募集。通过避免卵巢卵泡储备的过早耗尽，AMH可能有助于优化母猪的繁殖性能。
Anti-Müllerian hormone (AMH) is expressed by granulosa cells of developing follicles and plays an inhibiting role in the cyclic process of follicular recruitment by determining follicle-stimulating hormone threshold levels. Knowledge of AMH expression in the porcine ovary is important to understand the reproductive efficiency in female pigs. In the present study we investigated the expression of AMH during follicular development in prepubertal and adult female pigs by immunohistochemistry, laser capture micro-dissection and RT-qPCR. Although in many aspects the immunohistochemical localization of AMH in the porcine ovary does not differ from other species, there are also some striking differences. As in most species, AMH appears for the first time during porcine follicular development in the fusiform granulosa cells of recruited primordial follicles and continues to be present in granulosa cells up to the antral stage. By the time follicles reach the pre-ovulatory stage, AMH staining intensity increases significantly, and both protein and gene expression is not restricted to granulosa cells; theca cells now also express AMH. AMH continues to be expressed after ovulation in the luteal cells of the corpus luteum, a phenomenon unique to the porcine ovary. The physiological function of AMH in the corpus luteum is at present not clear. One can speculate that it may contribute to the regulation of the cyclic recruitment of small antral follicles. By avoiding premature exhaustion of the ovarian follicular reserve, AMH may contribute to optimization of reproductive performance in female pigs.
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影响因子：
4.8
作者：
Durlinger, ALL;Gruijters, MJG;Themmen, APN
通讯作者：
Themmen, APN
影响因子：
6.7
作者：
Chang, Hsun-Ming;Klausen, Christian;Leung, Peter C. K.
通讯作者：
Leung, Peter C. K.
影响因子：
6.1
作者：
Carlsson, I. B.;Scott, J. E.;Hovatta, O.
通讯作者：
Hovatta, O.
DOI：
10.1530/jrf.0.0800509
发表时间：
1987-07-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
BEZARD, J;VIGIER, B;JOSSO, N
通讯作者：
JOSSO, N
影响因子：
6.7
作者：
Botkjaer, Jane Alro;Jeppesen, Janni Vikkelso;Andersen, Claus Yding
通讯作者：
Andersen, Claus Yding