High-throughput flow alignment of barcoded hydrogel microparticles.
High-throughput flow alignment of barcoded hydrogel microparticles.
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DOI：
10.1039/b909959j
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发表时间：
2009-11-07
期刊：
影响因子：
6.1
通讯作者：
Doyle PS
中科院分区：
文献类型：
--
作者：
Chapin SC;Pregibon DC;Doyle PS
悬浮（基于颗粒的）阵列在生物分子的检测和定量方面提供了优于常规平面阵列的几个优点，包括使用更小的样品体积、更有利的探针-靶结合动力学和快速的探针组修饰。我们提出了一种用于多功能水凝胶微粒的快速对齐的微流控系统，所述多功能水凝胶微粒设计成承载一个或多个生物分子探针区域，以及用于识别嵌入的探针的图形代码。使用高速成像，我们开发并优化了一种流通系统，该系统（1）允许高颗粒通量，（2）确保解码和目标定量的适当颗粒对齐，以及（3）可以可靠地连续操作而不会堵塞。侧面为侧聚焦流的锥形通道用于将柔性片状颗粒定向成通道中心的有序流动。探索通道几何形状、颗粒几何形状、颗粒组成、颗粒装载密度和条形码设计的影响，以确定最终用于生物测定的最佳组合。优化系统中的粒子以约50 cm s−1的速度移动，吞吐量约为40个粒子s−1。简单的物理模型和计算流体力学模拟已被用来研究在设备中的流动行为。
Suspension (particle-based) arrays offer several advantages over conventional planar arrays in the detection and quantification of biomolecules, including the use of smaller sample volumes, more favorable probe-target binding kinetics, and rapid probe-set modification. We present a microfluidic system for the rapid alignment of multifunctional hydrogel microparticles designed to bear one or several biomolecule probe regions, as well as a graphical code to identify the embedded probes. Using high-speed imaging, we have developed and optimized a flow-through system that (1) allows for a high particle throughput, (2) ensures proper particle alignment for decoding and target quantification, and (3) can be reliably operated continuously without clogging. A tapered channel flanked by side focusing streams is used to orient the flexible, tablet-shaped particles into a well-ordered flow in the center of the channel. The effects of channel geometry, particle geometry, particle composition, particle loading density, and barcode design are explored to determine the best combination for eventual use in biological assays. Particles in the optimized system move at velocities of ~50 cm s−1 and with throughputs of ~40 particles s−1. Simple physical models and CFD simulations have been used to investigate flow behavior in the device.
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影响因子：
8.6
作者：
Morgan, E;Varro, R;Gaur, A
通讯作者：
Gaur, A
DOI：
10.1098/rspb.1969.0088
发表时间：
1969-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FITZGERALD, JM
通讯作者：
FITZGERALD, JM
影响因子：
3.7
作者：
HALPERN, D;SECOMB, TW
通讯作者：
SECOMB, TW
影响因子：
12.3
作者：
Peck, David;D Crawford, Emily;Ross, Kenneth N.;Stegmaier, Kimberly;Golub, Todd R.;Lamb, Justin
通讯作者：
Lamb, Justin
影响因子：
6.1
作者：
Dendukuri, Dhananjay;Gu, Shelley S.;Doyle, Patrick S.
通讯作者：
Doyle, Patrick S.