Spaceflight Modifies Escherichia coli Gene Expression in Response to Antibiotic Exposure and Reveals Role of Oxidative Stress Response.
Spaceflight Modifies Escherichia coli Gene Expression in Response to Antibiotic Exposure and Reveals Role of Oxidative Stress Response.
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DOI：
10.3389/fmicb.2018.00310
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发表时间：
2018
影响因子：
5.2
通讯作者：
Chatterjee A
中科院分区：
文献类型：
--
作者：
Aunins TR;Erickson KE;Prasad N;Levy SE;Jones A;Shrestha S;Mastracchio R;Stodieck L;Klaus D;Zea L;Chatterjee A
在微重力条件下的空间实验中生长的细菌被发现经历了独特的生理反应，从改变细胞形态和生长动力学到可能增加对抗生素的耐受性。关于这种行为的一种常见理论是，轨道环境中重力驱动的对流过程的丧失，导致细胞外养分供应的减少和细菌副产物在细胞附近的积累。为了进一步表征这些反应，本研究调查了大肠杆菌对微重力和抗生素浓度的转录反应。在国际空间站上，在抗生素庆大霉素浓度不断增加的情况下，通过在地球上进行相同的地面控制，培养出了大肠杆菌。在这里，我们表明，在培养的49小时内，大肠杆菌适应了太空中比地球上更高的抗生素浓度，并显示出63个基因的表达随着两种环境中药物浓度的增加而发生一致的变化，包括与氧化应激和饥饿反应相关的特异性反应。此外，我们还发现，与地面对照中的同等浓度相比，有50个胁迫反应基因在微重力下上调。我们得出结论，微重力下抗生素耐受性的增加可能不仅归因于运输过程的减少，而且还归因于应激反应基因的重叠效应所产生的抗生素交叉耐药反应。我们的数据表明，微重力环境传递的营养饥饿和酸休克的直接应激可以顺便上调与抗生素应激相关的应激反应途径，从而有助于在空间实验中观察到细菌对抗生素应激耐受性的提高。这些结果为细菌在极端压力条件下的适应能力以及在太空和地球上防止抗菌素耐药性的潜在战略提供了见解。
Bacteria grown in space experiments under microgravity conditions have been found to undergo unique physiological responses, ranging from modified cell morphology and growth dynamics to a putative increased tolerance to antibiotics. A common theory for this behavior is the loss of gravity-driven convection processes in the orbital environment, resulting in both reduction of extracellular nutrient availability and the accumulation of bacterial byproducts near the cell. To further characterize the responses, this study investigated the transcriptomic response of Escherichia coli to both microgravity and antibiotic concentration. E. coli was grown aboard International Space Station in the presence of increasing concentrations of the antibiotic gentamicin with identical ground controls conducted on Earth. Here we show that within 49 h of being cultured, E. coli adapted to grow at higher antibiotic concentrations in space compared to Earth, and demonstrated consistent changes in expression of 63 genes in response to an increase in drug concentration in both environments, including specific responses related to oxidative stress and starvation response. Additionally, we find 50 stress-response genes upregulated in response to the microgravity when compared directly to the equivalent concentration in the ground control. We conclude that the increased antibiotic tolerance in microgravity may be attributed not only to diminished transport processes, but also to a resultant antibiotic cross-resistance response conferred by an overlapping effect of stress response genes. Our data suggest that direct stresses of nutrient starvation and acid-shock conveyed by the microgravity environment can incidentally upregulate stress response pathways related to antibiotic stress and in doing so contribute to the increased antibiotic stress tolerance observed for bacteria in space experiments. These results provide insights into the ability of bacteria to adapt under extreme stress conditions and potential strategies to prevent antimicrobial-resistance in space and on Earth.
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DOI：
10.1093/bioinformatics/btn142
发表时间：
2008-07-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Ho JW;Stefani M;dos Remedios CG;Charleston MA
通讯作者：
Charleston MA
影响因子：
1.8
作者：
Brown, RB;Klaus, D;Todd, P
通讯作者：
Todd, P
影响因子：
2.8
作者：
Klaus, D;Simske, S;Stodieck, L
通讯作者：
Stodieck, L
影响因子：
3.6
作者：
Hirakawa, H;Inazumi, Y;Yamaguchi, A
通讯作者：
Yamaguchi, A
影响因子：
2.1
作者：
Fuentes, AM;Díaz-Mejía, JJ;Amábile-Cuevas, CF
通讯作者：
Amábile-Cuevas, CF