Ultrastructural studies of late-stage spermatids and mature spermatozoa of the puffer fish, Takifugu niphobles (Tetraodontiformes) and the effects of osmolality on spermatozoan structure.
Ultrastructural studies of late-stage spermatids and mature spermatozoa of the puffer fish, Takifugu niphobles (Tetraodontiformes) and the effects of osmolality on spermatozoan structure.
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河豚、河鲀后期精子细胞和成熟精子的超微结构研究以及渗透压对精子结构的影响。
DOI：
10.1054/tice.2000.0159
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发表时间：
2001
期刊：
影响因子：
2.6
通讯作者：
S. Morisawa
中科院分区：
文献类型：
--
作者：
S. Morisawa
研究了不同渗透压条件下河豚晚期精子和精子的超微结构。成熟精子由一个精子头、多个线粒体的中间部分和具有尖尖的鞭毛组成，在细胞质套质膜内侧有许多环状结构，鞭毛基部有突出到细胞质管的三角形结构。晚期精子细胞有环和突起，但侧鳍末端为圆形，鞭毛细胞质无定形。当精子暴露在海水中时，质膜在头部中部区域肿胀，而在尾部区域收缩。在1/2海水中，部分精子出现尾部肿胀。在1/3与精浆近似等渗的海水中，变化不大。在1/10的海水中，头部区域的质膜有轻微的肿胀，尾部区域的质膜肿胀明显。在缓冲液中，所有区域的膜都膨胀，包围着细胞核和轴突的许多部分。因此，质膜在头部区域的功能可能与尾部区域的功能不同。当精子周围的渗透压由于精浆与海水的混合而发生变化，达到使精子获得正确结构的适当水平时，海洋鱼类的精子才能使卵子受精。
Ultrastructures of late-stage spermatids and spermatozoa, and of spermatozoa after exposure to various osmolalities, were studied in the puffer, Takifugu niphobles. The mature spermatozoa consisted of a head, a midpiece of many mitochondria and a flagellum with sharp sidefins, had many ring-structures just inside of the plasma membrane of cytoplasmic sleeve and triangular-structures projecting into cytoplasmic canal at the base of flagellum. In late spermatids, the rings and projections were present, but the side-fins had round ends and the cytoplasm of flagellum was amorphous. When spermatozoa were exposed to seawater, the plasma membrane became swollen in the head-midpiece region but shrank in the tail region. In 1/2 seawater, swelling in the tail occurred in some spermatozoa. In 1/3 seawater approximately isotonic to the seminal plasma, there was little change. In 1/10 seawater, the plasma membrane swelled slightly in the head region, but swelled much more in the tail region. In buffer solution, the membrane swelled in all regions, surrounding the nucleus and many sections of axoneme. Thus, function of the plasma membrane in the head-region may be different from that in the tail-region. Spermatozoa of marine fish may fertilize the eggs when the osmolality surrounding the sperm, which changes due to the mixing of seminal plasma and seawater, reaches the correct level for the spermatozoa to obtain correct structure.
DOI：
10.1002/aja.1001780109
发表时间：
1987
期刊：
The American journal of anatomy
影响因子：
--
作者：
Robert L. Sprando;Lonnie D. Russell;Lonnie D. Russell
通讯作者：
Robert L. Sprando;Lonnie D. Russell;Lonnie D. Russell