Genome-resolved metagenomic analysis reveals different functional potentials of multiple Candidatus Brocadia species in a full-scale swine wastewater treatment system
Genome-resolved metagenomic analysis reveals different functional potentials of multiple Candidatus Brocadia species in a full-scale swine wastewater treatment system
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基因组解析宏基因组分析揭示了多种 Candidatus Brocadia 物种在全规模猪废水处理系统中的不同功能潜力
DOI：
10.1007/s11783-023-1602-7
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发表时间：
2022-07
期刊：
影响因子：
--
通讯作者：
Fangang Meng
中科院分区：
文献类型：
--
作者：
Yabing Meng;Depeng Wang;Zhong Yu;Qingyun Yan;Zhili He;Fangang Meng
厌氧氨氧化工艺的应用越来越广泛，表明其在污水处理设施中脱氮方面具有巨大的潜力。然而，在复杂生态系统中共存的厌氧氨氧化菌物种的功能潜力和生态分化还没有得到很好的阐明。在此，通过利用功能重建和比较基因组分析，我们破译了四种的共同出现的机制，自发地丰富了一个全面的猪污水处理系统。系统发育分析表明，SW172和SW745与Ca. Brocadia caroliniensis和Ca.优势种SW 510和SW 773的总平均多度为34.1%，被列为该属的新种。布罗卡迪亚功能重建表明，新物种SW510可以编码细胞色素cd1型亚硝酸盐还原酶和羟胺氧化酶的亚硝酸盐还原。相反，检测到的呼吸五血红素细胞色素c亚硝酸盐还原酶和乙酸激酶基因表明，SW773可能以乙酸盐为碳源将亚硝酸盐还原为铵。有趣的是，编码尿素酶和氰化酶的基因的存在表明，这两个新物种除了氨和亚硝酸盐作为底物外，还可以使用多种有机氮化合物。总之，这些anammox基因组的恢复和比较分析扩大了我们对Ca属的功能分化和共存的理解。污水处理系统中的布罗卡迪亚。
The increasing application of anammox processes suggests their enormous potential for nitrogen removal in wastewater treatment facilities. However, the functional potentials and ecological differentiation of cooccurring anammox species in complex ecosystems have not been well elucidated. Herein, by utilizing functional reconstruction and comparative genome analysis, we deciphered the cooccurring mechanisms of four Candidatus Brocadia species that were spontaneously enriched in a full-scale swine wastewater treatment system. Phylogenetic analysis indicated that species SW172 and SW745 were closely related to Ca. Brocadia caroliniensis and Ca. Brocadia sapporoensis, respectively, whereas the dominant species SW510 and SW773, with a total average abundance of 34.1%, were classified as novel species of the genus Ca. Brocadia. Functional reconstruction revealed that the novel species SW510 can encode both cytochrome cd1-type nitrite reductase and hydroxylamine oxidase for nitrite reduction. In contrast, the detected respiratory pentaheme cytochrome c nitrite reductase and acetate kinase genes suggested that SW773 likely reduced nitrite to ammonium with acetate as a carbon source. Intriguingly, the presence of genes encoding urease and cyanase indicated that both novel species can use diverse organic nitrogen compounds in addition to ammonia and nitrite as substrates. Taken together, the recovery and comparative analysis of these anammox genomes expand our understanding of the functional differentiation and cooccurrence of the genus Ca. Brocadia in wastewater treatment systems.
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影响因子：
12.8
作者：
Hongkeun Park;A. C. Brotto;M. V. van Loosdrecht;K. Chandran
通讯作者：
Hongkeun Park;A. C. Brotto;M. V. van Loosdrecht;K. Chandran
影响因子：
6.4
作者：
Q. Zheng;Minglu Zhang;Tingting Zhang;Xinhui Li;Minghan Zhu;Xiaohui Wang
通讯作者：
Q. Zheng;Minglu Zhang;Tingting Zhang;Xinhui Li;Minghan Zhu;Xiaohui Wang
DOI：
10.1016/0003-2697(90)90598-4
发表时间：
1971
期刊：
--
影响因子：
--
作者：
D. Washington
通讯作者：
D. Washington
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
6.4
作者：
Fengsheng Hou;Ting Zhang;Yongzhen Peng;Xiaoxin Cao;Hongtao Pang;Y. Shao;Xianchun Lu;Juan-fa Yuan-Ju
通讯作者：
Fengsheng Hou;Ting Zhang;Yongzhen Peng;Xiaoxin Cao;Hongtao Pang;Y. Shao;Xianchun Lu;Juan-fa Yuan-Ju