Dynamic Acclimation to High Light in Arabidopsis thaliana Involves Widespread Reengineering of the Leaf Proteome
Dynamic Acclimation to High Light in Arabidopsis thaliana Involves Widespread Reengineering of the Leaf Proteome
复制标题
拟南芥对强光的动态适应涉及叶片蛋白质组的广泛重组
DOI：
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发表时间：
2017
影响因子：
5.6
通讯作者：
G. Johnson
中科院分区：
文献类型：
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作者：
Matthew A E Miller;Ronan O'Cualain;Julian N. Selley;David R. Knight;M. F. Karim;S. Hubbard;G. Johnson
拟南芥叶片从弱光到强光的转换增加了它们的光合作用能力，这是一个动态的适应过程。一个突变体，gpt 2，缺乏叶绿体葡萄糖-6-磷酸/磷酸转运蛋白，是缺乏适应增加的光的能力。在这里，我们使用了一个无标记的蛋白质组学方法，进行相对定量的1993年蛋白质从拟南芥野生型和gpt 2叶片暴露于增加的光。数据可通过ProteomeXchange获得，标识符为PXD 006598。光驯化涉及电子传递和碳代谢的增加，但在光合反应中心的丰度没有变化。gpt 2突变体显示出与野生型相似的总蛋白含量增加，但某些蛋白的变化程度不同，包括细胞色素b6 f复合物和质体蓝素、类囊体ATP酶和选定的Benson-Calvin循环酶的相对丰度。植物适应环境后叶中代谢物含量的变化可以用参与代谢的酶的丰度变化来解释，在某些情况下，gpt 2中这些酶的丰度降低。gpt 2的植物在胁迫相关蛋白中投入更多，这表明它们适应光合能力的能力降低导致胁迫增加。
Leaves of Arabidopsis thaliana transferred from low to high light increase their capacity for photosynthesis, a process of dynamic acclimation. A mutant, gpt2, lacking a chloroplast glucose-6-phosphate/phosphate translocator, is deficient in its ability to acclimate to increased light. Here, we have used a label-free proteomics approach, to perform relative quantitation of 1993 proteins from Arabidopsis wild type and gpt2 leaves exposed to increased light. Data are available via ProteomeXchange with identifier PXD006598. Acclimation to light is shown to involve increases in electron transport and carbon metabolism but no change in the abundance of photosynthetic reaction centers. The gpt2 mutant shows a similar increase in total protein content to wild type but differences in the extent of change of certain proteins, including in the relative abundance of the cytochrome b6f complex and plastocyanin, the thylakoid ATPase and selected Benson-Calvin cycle enzymes. Changes in leaf metabolite content as plants acclimate can be explained by changes in the abundance of enzymes involved in metabolism, which were reduced in gpt2 in some cases. Plants of gpt2 invest more in stress-related proteins, suggesting that their reduced ability to acclimate photosynthetic capacity results in increased stress.
影响因子：
6.5
作者：
Nunes, Catia;Primavesi, Lucia F.;Paul, Matthew J.
通讯作者：
Paul, Matthew J.
影响因子：
4.4
作者：
Serang O;Cansizoglu AE;Käll L;Steen H;Steen JA
通讯作者：
Steen JA