Field-free transmission geometry atmospheric pressure matrix-assisted laser desorption/ionization for rapid analysis of unadulterated tissue samples.
Field-free transmission geometry atmospheric pressure matrix-assisted laser desorption/ionization for rapid analysis of unadulterated tissue samples.
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无场传输几何大气压矩阵辅助激光解吸/电离，用于快速分析纯组织样本。
DOI：
10.1002/rcm.4213
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
J. Michael Walker
中科院分区：
文献类型：
--
作者：
S. Trimpin;Thushani N. Herath;Ellen D. Inutan;S. Alexandru Cernat;James B. Miller;Ken Mackie;J. Michael Walker
组织成像在检测癌症边界和绘制脑组织中的信号分子等领域具有巨大的应用潜力。为了保留有关组织分子组成的位置和组装的信息，材料应该尽可能保持不掺杂。样品的制备和采集条件，特别是使用溶剂和真空时，1可能会变得不利。在这里，我们报告了使用无场(FF)传输几何(TG)大气压(AP)源设计的无溶剂基质辅助激光解吸/电离(MALDI)质谱仪(MS)分析小鼠脑组织。用MS进行成像已经很成熟了，特别是使用二次离子质谱仪(SIMS)，但它对完整的生物组织只有一点点用处。然而，MALDI MS已经成功地用于组织成像，特别是高丰度组分，如膜脂类、药物代谢物和蛋白质。2常规实现100 mm的空间分布。然而，使用基于真空的MS进行组织成像有许多缺点，特别是对于未掺杂的组织(图1)。AP MALDI组织成像避开了真空MALDI的许多缺点，但由于敏感性问题而受到限制。3解吸电喷雾电离(DESI)是一种由AP的液体喷雾产生的离子，目前正在探索作为一种组织成像方法，但目前可用的分辨率(200 Mm)受到许多因素的限制。4质量特异性组织成像的改进将导致许多生物学应用。与MALDI MS成像相关的主要挑战包括MALDI基质沉积时空间分辨率的降低，这些复杂样品中大量化合物造成的干扰和抑制，包括盐的自然丰度，检测灵敏度不足，纯基于MS的分析的有限的动态范围，以及与将组织置于真空中相关的问题。定量是有问题的，此外，基质背景可能会干扰小分子(如药物)的MALDI分析。尽管MALDI MS具有极高的固有灵敏度，5各种生物化合物的复杂性和浓度范围
Tissue imaging has the potential to be immensely useful in areas as diverse as detecting cancer boundaries and mapping signaling molecules in brain tissue. In order to retain the information on the location and assembly of the molecular makeup of tissue, the materials should stay unadulterated to the largest extent possible. The sample preparation and acquisition conditions, especially the use of solvent and vacuum, 1 may become detrimental. Here, we report the analysis of mouse brain tissue by solvent-free matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) using a field-free (FF) transmission geometry (TG) atmospheric pressure (AP) source design. Imaging by MS is well established, especially using secondary ion mass spectrometry (SIMS), but it is only marginally useful with intact biological tissue. MALDI MS, however, has been employed for tissue imaging with success, especially for high-abundance components such as membrane lipids, drug metabolites, and proteins. 2 Spatial distributions of 100 mm are routinely achieved. 2 However, there are a number of disadvantages in using vacuum-based MS for tissue imaging, especially in relation to unadulterated tissue (Fig. 1). AP MALDI tissue imaging circumvents many of the disadvantages of vacuum MALDI but has been limited because of sensitivity issues. 3 Desorption electrospray ionization (DESI), in which ions are produced by a liquid spray at AP, is currently being explored as a method for tissue imaging, but the resolution currently available (200mm) is limited by a number of factors. 4 Improvements in mass-specific tissue imaging would lead to numerous biological applications.Major challenges associated with imaging by MALDI MS include the degradation of the spatial resolution upon deposition of the MALDI matrix, interferences and suppression caused by the multitude of compounds in these complex samples including the natural abundance of salts, insufficient detection sensitivity, the limited dynamic range of a purely MS-based analysis and problems associated with placing tissue in vacuum. Quantitation is problematic and, in addition, the matrix background can interfere in the MALDI analysis of small molecules (eg drugs). In spite of the superb inherent sensitivity of MALDI MS, 5 the complexity and concentration range of various biological compounds in
影响因子：
7.4
作者：
Baluya, Dodge L.;Garrett, Timothy J.;Yost, Richard A.
通讯作者：
Yost, Richard A.
通过串联质谱法鉴定牛中分子量和低分子量神经丝蛋白的内源磷酸化位点。
影响因子：
2.9
作者：
Trimpin,Sarah;Mixon,AprilE;Stapels,MarthaD;Kim,Moo-Young;Spencer,PeterS;Deinzer,MaxL
通讯作者：
Deinzer,MaxL