Rapid Communication: Somatic Cell Nuclear Transfer in Humans: Pronuclear and Early Embryonic Development
Rapid Communication: Somatic Cell Nuclear Transfer in Humans: Pronuclear and Early Embryonic Development
复制标题
快速通讯：人类体细胞核移植：原核和早期胚胎发育
DOI：
10.1089/152489001753262168
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. West
中科院分区：
文献类型：
--
作者：
J. Cibelli;A. Kiessling;K. Cunniff;C. Richards;R. Lanza;M. West
人的治疗性克隆需要通过核移植对体细胞进行重编程，以产生自体全能干细胞。我们已经孤雌激活了22个人类卵子，并在17个中期II卵子中进行了核移植。在孤雌激活卵中获得了超过8个细胞阶段的卵裂，并在6个中观察到囊胚腔。三个体细胞衍生的胚胎发育超过原核阶段的六细胞阶段。创造自体胚胎的能力代表了产生免疫相容干细胞的第一步，这些干细胞可用于克服再生医学中的免疫排斥问题。
Human therapeutic cloning requires the reprogramming of a somatic cell by nuclear transfer to generate autologous totipotent stem cells. We have parthenogenetically activated 22 human eggs and also performed nuclear transfer in 17 metaphase II eggs. Cleavage beyond the eight-cell stage was obtained in the parthenogenetic-activated eggs, and blastocoele cavities were observed in six. Three somatic cell-derived embryos developed beyond the pronuclear stage up to the six-cell stage. The ability to create autologous embryos represents the first step towards generating immune-compatible stem cells that could be used to overcome the problem of immune rejection in regenerative medicine.
影响因子：
15.9
作者：
Klug, MG;Soonpaa, MH;Field, LJ
通讯作者：
Field, LJ
通过将培养的胎儿成纤维细胞显微注射到体外成熟卵母细胞中重建猪胚胎的发育。
DOI：
10.1016/s0378-4320(99)00037-8
发表时间：
1999
期刊：
Animal reproduction science.
影响因子：
--
作者：
Tao,T;Machaty,Z;Boquest,AC;Day,BN;Prather,RS
通讯作者：
Prather,RS
影响因子：
56.9
作者：
Brüstle, O;Jones, KN;McKay, RDG
通讯作者：
McKay, RDG