Costunolide Influences Germ Tube Orientation in Sunflower Broomrape - A First Step Toward Understanding Chemotropism.
Costunolide Influences Germ Tube Orientation in Sunflower Broomrape - A First Step Toward Understanding Chemotropism.
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Costunolide影响葵花籽扫帚中的细菌管取向 - 了解趋化性的第一步。
DOI：
10.3389/fpls.2021.699068
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发表时间：
2021
影响因子：
5.6
通讯作者：
Spring O
中科院分区：
文献类型：
--
作者：
Krupp A;Bertsch B;Spring O
列当属库马纳是栽培向日葵的宿主特异性根寄生物，在欧洲、北非和亚洲部分地区具有越来越重要的经济意义。向日葵根中释放的倍半萜内酯类化合物是O.库马纳种子在土壤中，化学性质的信号引导新兴的芽管向主机根至今仍是未知的。因此，我们设计了一种生物测定，允许观察肉苁蓉发芽和随后的芽管发育的物质存在下，推定的趋化活性。根分泌物和向日葵油提取物，都含有微摩尔浓度的STLs，导致芽管的正向化性取向。一个类似的积极的趋化作用，实现了与costuntoluene，四个STL的向日葵存在于渗出液和油提取物。与此相反，GR 24，一个人工合成的独脚金内酯（SL）对O。库马纳种子，没有表现出对芽管方向的影响。木香酮的效果是浓度依赖性的，并在其自然微摩尔发生在根部的范围内。我们假设，STL梯度是负责较强的抑制伸长生长的主机面向侧翼的芽管相比，远侧侧翼。这将赋予双重作用的向日葵根分泌物中的向日葵，肉苁蓉的相互作用，即，作为发芽刺激剂和趋化信号的STL。
Orobanche cumana WALLR. is a host-specific root parasite of cultivated sunflowers with increasing economic importance in Europe, North Africa, and parts of Asia. While sesquiterpene lactones (STLs) released from sunflower roots were identified as natural germination stimulants of O. cumana seeds in the soil, the chemical nature of the signals guiding the emerging germ tube toward the host root has remained unknown hitherto. Thus, we designed a bioassay that allowed the observation of broomrape germination and subsequent germ tube development in the presence of substances with putative chemotropic activity. Root exudates and sunflower oil extracts, both containing STLs in micromolar concentrations, caused the positive chemotropic orientation of germ tubes. A similar positive chemotropic effect was achieved with costunolide, one of the four STLs of sunflower present in the exudate and oil extracts. In contrast, GR24, a synthetic strigolactone (SL) with germination-inducing activity on O. cumana seeds, showed no effect on the germ tube orientation. The effect of costunolide was concentration-dependent and within the range of its natural micromolar occurrence in roots. We assume that an STL gradient is responsible for the stronger inhibition of elongation growth on the host-facing flank of the germ tube compared with the far side flank. This would confer a double role of STLs from sunflower root exudates in the sunflower–broomrape interaction, namely, as germination stimulants and as chemotropic signals.
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影响因子：
4.3
作者：
Spring, Otmar;Schmauder, Katharina;Frey, Maximilian
通讯作者：
Frey, Maximilian
DOI：
10.1139/b94-075
发表时间：
1994-05-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
JOEL, DM;LOSNERGOSHEN, D
通讯作者：
LOSNERGOSHEN, D
影响因子：
3.8
作者：
Joel, Daniel M.;Chaudhuri, Swapan K.;Steffens, John C.
通讯作者：
Steffens, John C.
影响因子：
4.3
作者：
SPRING, O;HAGER, A
通讯作者：
HAGER, A
影响因子：
2.5
作者：
Dor, Evgenia;Plakhine, Dina;Hershenhorn, Joseph
通讯作者：
Hershenhorn, Joseph