Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.
Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.
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DOI：
10.1016/j.celrep.2022.110411
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发表时间：
2022-03-08
期刊：
影响因子：
8.8
通讯作者：
Gewurz BE
中科院分区：
文献类型：
--
作者：
Yiu SPT;Guo R;Zerbe C;Weekes MP;Gewurz BE
Epstein-Barr virus (EBV) persistently infects people worldwide. Delivery of ~170-kb EBV genomes to nuclei and use of nuclear membrane-less replication compartments (RCs) for their lytic cycle amplification necessitate evasion of intrinsic antiviral responses. Proteomics analysis indicates that, upon B cell infection or lytic reactivation, EBV depletes the cohesin SMC5/6, which has major roles in chromosome maintenance and DNA damage repair. The major tegument protein BNRF1 targets SMC5/6 complexes by a ubiquitin proteasome pathway dependent on calpain proteolysis and Cullin-7. In the absence of BNRF1, SMC5/6 associates with R-loop structures, including at the viral lytic origin of replication, and interferes with RC formation and encapsidation. CRISPR analysis identifies RC restriction roles of SMC5/6 components involved in DNA entrapment and SUMOylation. Our study highlights SMC5/6 as an intrinsic immune sensor and restriction factor for a human herpesvirus RC and has implications for the pathogenesis of EBV-associated cancers. Yiu et al. identify that Epstein-Barr virus subverts the SMC5/6 cohesin complex upon B cell infection and lytic reactivation. The viral tegument protein BNRF1 targets SMC5/6 for proteasomal degradation via Cullin 7 and calpain. Without BNRF1, SMC5/6 can sense viral genomic R-loops and prevent formation of lytic replication compartments.
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影响因子：
5.4
作者：
Feng, WH;Hong, G;Kenney, SC
通讯作者：
Kenney, SC
影响因子：
9.8
作者：
Altmann, Markus;Hammerschmidt, Wolfgang
通讯作者：
Hammerschmidt, Wolfgang
DOI：
10.3390/pathogens9100786
发表时间：
2020-09-25
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Gibson RT;Androphy EJ
通讯作者：
Androphy EJ
影响因子：
5.4
作者：
Calderwood, Michael A.;Holthaus, Amy M.;Johannsen, Eric
通讯作者：
Johannsen, Eric
DOI：
10.4049/jimmunol.1301629
发表时间：
2013-12-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Abbott RJ;Quinn LL;Leese AM;Scholes HM;Pachnio A;Rickinson AB
通讯作者：
Rickinson AB