Epithelial-to-mesenchymal transition leads to loss of EpCAM and different physical properties in circulating tumor cells from metastatic breast cancer.
Epithelial-to-mesenchymal transition leads to loss of EpCAM and different physical properties in circulating tumor cells from metastatic breast cancer.
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DOI：
10.18632/oncotarget.8250
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发表时间：
2016-04-26
期刊：
影响因子：
--
通讯作者：
Kim YS
中科院分区：
文献类型：
--
作者：
Hyun KA;Koo GB;Han H;Sohn J;Choi W;Kim SI;Jung HI;Kim YS
循环肿瘤细胞（CTC）的传播需要上皮-间充质转化（EMT），其中细胞失去其上皮特征并获得更多间充质样表型。目前CTC的分离依赖于依赖于上皮细胞粘附分子（EpCAM）表达的基于亲和力的方法。在这里，我们显示EMT诱导的乳腺癌细胞保持在长期的乳腺球培养条件下具有增加的EMT标志物和癌症干细胞标志物，以及减少的细胞质量和大小通过定量相显微镜;然而，EpCAM表达显着降低在这些细胞。此外，使用无标记微流体流动分级装置从乳腺癌患者分离的CTC具有不同的EpCAM表达模式，表明依赖于EpCAM表达的亲和方法可能低估CTC数量并可能错过关键亚群。使用该技术进一步表征CTC，包括低EpCAM群体，可以改善检测技术和癌症诊断，最终改善癌症治疗。
The dissemination of circulating tumor cells (CTCs) requires the Epithelial-to-Mesenchymal transition (EMT), in which cells lose their epithelial characteristics and acquire more mesenchymal-like phenotypes. Current isolation of CTCs relies on affinity-based approaches reliant on the expression of Epithelial Cell Adhesion Molecule (EpCAM). Here we show EMT-induced breast cancer cells maintained in prolonged mammosphere culture conditions possess increased EMT markers and cancer stem cell markers, as well as reduced cell mass and size by quantitative phase microscopy; however, EpCAM expression is dramatically decreased in these cells. Moreover, CTCs isolated from breast cancer patients using a label-free microfluidic flow fractionation device had differing expression patterns of EpCAM, indicating that affinity approaches reliant on EpCAM expression may underestimate CTC number and potentially miss critical subpopulations. Further characterization of CTCs, including low-EpCAM populations, using this technology may improve detection techniques and cancer diagnosis, ultimately improving cancer treatment.
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