Soil bacteria protect fungi from phenazines by acting as toxin sponges.
Soil bacteria protect fungi from phenazines by acting as toxin sponges.
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DOI：
10.1016/j.cub.2021.11.002
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发表时间：
2022-01-24
期刊：
影响因子：
--
通讯作者：
Newman DK
中科院分区：
文献类型：
--
作者：
Dahlstrom KM;Newman DK
许多环境和临床上重要的真菌对称为苯吡啶的有毒，生产的氧化还原活性分子敏感。尽管真菌容易受到苯丙胺攻击的影响，但居住在含有苯嗪生产商的微生物群落中。由于许多真菌无法承受苯嗪的挑战，但是一些细菌物种可以承受，我们假设细菌伴侣可以保护苯嗪恢复环境中的真菌。从单个土壤样品中，我们能够共同分解几种这种与物理相关的配对。我们发现了新型物种paraburkholderia edwinii，并证明它可以保护共隔离的曲霉物种免受苯丙胺-1-羧酸（PCA）的侵害，并通过将其隔离为毒素海绵。反过来，它还获得了保护。当受到PCA的挑战时，P。Edwinii会改变其形态，从而在不断增长的真菌菌落中形成聚集体。此外，真菌伴侣触发了P. Edwinii，以隔离PCA，并通过促进缺氧和高度减少的环境来维持限制PCA毒性的条件。 P. Edwinii的诱变屏幕揭示了该保护程序取决于应力诱导的转录阻遏物HRCA。我们表明，应对PCA挑战的一种相关应激源是真菌酸化，酸应激会导致埃德维尼（P. edwinii）的行为，好像存在真菌一样。最后，我们揭示了这种现象是在细菌和真菌伴侣（包括植物和人类病原体）中具有适度特异性的Paraburkholderia中普遍存在的。我们的发现提出了一种常见的机制，真菌可以通过该机制进入苯嗪恢复环境，并为其研究提供了可拖动的模型系统。这些结果对根际以及植物和人类感染部位中的微生物群落如何通过化学辩证法谈判社区成员资格。
天然抗生素在微生物群落组成中起着关键作用，但是关于社区如何达到稳态会员资格知之甚少。达尔斯特罗姆（Dahlstrom）和纽曼（Newman）展示了对抗生素敏感的真菌如何通过作为毒素海绵的保护性细菌伴侣进入其他敌对环境。
Many environmentally and clinically important fungi are sensitive to toxic, bacterially-produced, redox-active molecules called phenazines. Despite being vulnerable to phenazine-assault, fungi inhabit microbial communities that contain phenazine producers. Because many fungi cannot withstand phenazine challenge, but some bacterial species can, we hypothesized that bacterial partners may protect fungi in phenazine-replete environments. From a single soil sample we were able to co-isolate several such physically associated pairings. We discovered the novel species Paraburkholderia edwinii and demonstrated it can protect a co-isolated Aspergillus species from phenazine-1-carboxylic acid (PCA) by sequestering it, acting as a toxin sponge; in turn, it also gains protection. When challenged with PCA, P. edwinii changes its morphology, forming aggregates within the growing fungal colony. Further, the fungal partner triggers P. edwinii to sequester PCA and maintains conditions that limit PCA toxicity by promoting an anoxic and highly reducing environment. A mutagenic screen of P. edwinii revealed this protective program depends on the stress-inducible transcriptional repressor HrcA. We show that one relevant stressor in response to PCA challenge is fungal acidification and that acid stress causes P. edwinii to behave as though the fungus were present. Finally, we reveal this phenomenon as widespread among Paraburkholderia with moderate specificity among bacterial and fungal partners, including plant and human pathogens. Our discovery suggests a common mechanism by which fungi can gain access to phenazine-replete environments, and provides a tractable model system for its study. These results have implications for how microbial communities in the rhizosphere as well as in plant and human infection sites negotiate community membership via a chemical dialectic. Natural antibiotics play a key role in microbial community composition, but little is known about how communities reach a steady-state membership. Dahlstrom and Newman demonstrate how antibiotic-sensitive fungi may gain access to otherwise hostile environments through a protective bacterial partner acting as a toxin sponge.
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影响因子：
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影响因子：
7.7
作者：
Dar D;Thomashow LS;Weller DM;Newman DK
通讯作者：
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