miR-375 inhibits the proliferation of gastric cancer cells by repressing ERBB2 expression.
miR-375 inhibits the proliferation of gastric cancer cells by repressing ERBB2 expression.
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miR-375 通过抑制 ERBB2 表达来抑制胃癌细胞的增殖。
DOI：
10.3892/etm.2014.1627
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发表时间：
2014-06
影响因子：
2.7
通讯作者：
Cao H
中科院分区：
文献类型：
--
作者：
Shen ZY;Zhang ZZ;Liu H;Zhao EH;Cao H
MicroRNA (miRNA) 是小的非编码 RNA 分子，以转录后方式调节靶基因的表达。越来越多的证据表明，miRNA 作为癌基因或抑癌基因，在癌症发病机制中发挥着重要作用，包括细胞凋亡、增殖和分化。此前，miR-375被证明与人类胃癌有关，但其机制仍知之甚少。在本研究中，miR-375被证明在胃癌组织中，特别是人表皮生长因子受体2（ERBB2）阳性的胃癌组织中表达下调。通过双荧光素酶测定和蛋白质印迹分析鉴定，ERBB2被证明是miR-375的靶基因。此外，miR-375过表达在体外抑制人胃癌细胞的增殖，并且ERBB2过表达可恢复抑制作用。因此，本研究结果表明miR-375通过靶向ERBB2与人胃癌发生相关。因此，miR-375可作为人胃癌潜在的临床分类标志物和治疗靶点。
MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate the expression of targeted genes in a post-transcriptional manner. Increasing evidence indicates that miRNAs play important roles in cancer pathogenesis, including apoptosis, proliferation and differentiation, as oncogenes or tumor suppressors. Previously, miR-375 was shown to be involved in human gastric cancer, however, the mechanism remains poorly understood. In the present study, miR-375 was shown to be downregulated in gastric cancer tissues, particularly human epidermal growth factor receptor 2 (ERBB2)-positive gastric cancer tissues. Identified by dual luciferase assays and western blot analysis, ERBB2 was demonstrated to be a target gene of miR-375. In addition, miR-375 overexpression suppressed the proliferation of human gastric cancer cells in vitro and the suppression effect was restored by ERBB2 overexpression. Thus, the results of the present study indicate that miR-375 is associated with human gastric carcinogenesis by targeting ERBB2. Therefore, miR-375 may be used as a potential clinical classification marker and therapeutic target for human gastric cancer.
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DOI：
10.1158/1078-0432.ccr-08-3131
发表时间：
2009-04-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Avissar M;Christensen BC;Kelsey KT;Marsit CJ
通讯作者：
Marsit CJ
影响因子：
168.9
作者：
Danaei, G;Vander Hoorn, S;Ezzati, M
通讯作者：
Ezzati, M
影响因子：
3.3
作者：
Park, Young Soo;Hwang, Hee Sang;Kang, Yoon-Koo
通讯作者：
Kang, Yoon-Koo
MiR-375 在胃癌中频繁下调，通过靶向 JAK2 抑制细胞增殖
影响因子：
44.1
作者：
Ding, Ling;Xu, Yanjun;Zhou, Tianhua
通讯作者：
Zhou, Tianhua
影响因子：
6.4
作者：
Fassan, Matteo;Mastracci, Luca;Rugge, Massimo
通讯作者：
Rugge, Massimo