TaNBP1, a guanine nucleotide-binding subunit gene of wheat, is essential in the regulation of N starvation adaptation via modulating N acquisition and ROS homeostasis.
TaNBP1, a guanine nucleotide-binding subunit gene of wheat, is essential in the regulation of N starvation adaptation via modulating N acquisition and ROS homeostasis.
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TaNBP1 是小麦的鸟嘌呤核苷酸结合亚基基因，通过调节氮获取和 ROS 稳态来调节氮饥饿适应。
DOI：
10.1186/s12870-018-1374-6
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发表时间：
2018-08-13
影响因子：
5.3
通讯作者：
Xiao K
中科院分区：
文献类型：
--
作者：
Liu Z;Zhao Y;Wang X;Yang M;Guo C;Xiao K
硝态氮（NO3−）是高等植物氮(N)的主要来源，除具有转导N信号的功能外。提高作物氮素利用效率已成为世界范围内促进可持续农业发展的有效策略。与氮素吸收相关的调控途径和相应的生化过程在很大程度上影响着植物对氮素饥饿的耐受性。因此，探索氮素利用效率（NUE）的分子机制和基因财富将为耐氮作物品种的分子育种铺平道路。在本研究中，我们鉴定了小麦（T. aestivum）鸟嘌呤核苷酸结合蛋白亚基β基因TaNBP1在介导植物氮饥饿反应中的功能。TaNBP1蛋白含有一个保守的W40结构域，并且TaNBP1- gfp（绿色荧光蛋白）信号集中在细胞质膜和细胞质溶胶的位置。在N饥饿胁迫下，TaNBP1转录本在根和叶中被诱导，这种上调表达通过N恢复处理得到恢复。相对于野生型，TaNBP1过表达可以改善植物的表型、扩大根系结构（RSA）和增加生物量，这与TaNBP1在促进N积累和改善活性氧（ROS）稳态中的作用有关。硝态氮转运蛋白（NRT）基因NtNRT2.2和抗氧化酶基因NtSOD1、NtSOD2和NtCAT1受TaNBP1的转录调控，参与植物氮素获取的改善和AE活性的提高。总之，TaNBP1是对氮饥饿胁迫的转录反应。该基因的过表达通过修改NRT基因NtNRT2.2和抗氧化酶基因NtSOD1、NtSOD2和NtCAT1的转录，通过改善N摄取和细胞ROS稳态来增强植物对N饥饿的适应。本研究有助于了解植物氮素饥饿反应的机制，以及在节氮栽培条件下提高氮素利用效率的转基因作物品种的效益。本文的在线版本（10.1186/s12870-018-1374-6）包含补充资料，授权用户可使用。
Nitrate (NO3−) is the major source of nitrogen (N) for higher plants aside from its function in transducing the N signaling. Improving N use efficiency of crops has been an effective strategy for promotion of the sustainable agriculture worldwide. The regulatory pathways associating with N uptake and the corresponding biochemical processes impact largely on plant N starvation tolerance. Thus, exploration of the molecular mechanism underlying nitrogen use efficiency (NUE) and the gene wealth will pave a way for molecular breeding of N starvation-tolerant crop cultivars. In the current study, we characterized the function of TaNBP1, a guanine nucleotide-binding protein subunit beta gene of wheat (T. aestivum), in mediating the plant N starvation response. TaNBP1 protein harbors a conserved W40 domain and the TaNBP1-GFP (green fluorescence protein) signals concentrate at positions of cytoplasm membrane and cytosol. TaNBP1 transcripts are induced in roots and leaves upon N starvation stress and that this upregulated expression is recovered by N recovery treatment. TaNBP1 overexpression confers improved phenotype, enlarged root system architecture (RSA), and increased biomass for plants upon N deprivation relative to the wild type, associating with its role in enhancing N accumulation and improving reactive oxygen species (ROS) homeostasis. Nitrate transporter (NRT) gene NtNRT2.2 and antioxidant enzyme genes NtSOD1, NtSOD2, and NtCAT1 are transcriptionally regulated under TaNBP1 and contribute to the improved N acquisition and the increased AE activities of plants. Altogether, TaNBP1 is transcriptional response to N starvation stress. Overexpression of this gene enhances plant N starvation adaptation via improvement of N uptake and cellular ROS homeostasis by modifying transcription of NRT gene NtNRT2.2 and antioxidant enzyme genes NtSOD1, NtSOD2, and NtCAT1, respectively. Our research helps to understand the mechanism underlying plant N starvation response and benefits to genetically engineer crop cultivars with improved NUE under the N-saving cultivation conditions. The online version of this article (10.1186/s12870-018-1374-6) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Lee S;Rojas CM;Ishiga Y;Pandey S;Mysore KS
通讯作者：
Mysore KS
影响因子：
7.4
作者：
Pandey, S;Chen, JG;Assmann, SM
通讯作者：
Assmann, SM
影响因子：
4.8
作者：
Heo, Jae Bok;Sung, Sibum;Assmann, Sarah M.
通讯作者：
Assmann, Sarah M.
通过改善中国集约农业系统的氮肥管理来降低环境风险
DOI：
10.1073/pnas.0813417106
发表时间：
2009-03-03
影响因子：
11.1
作者：
Ju, Xiao-Tang;Xing, Guang-Xi;Zhang, Fu-Suo
通讯作者：
Zhang, Fu-Suo
影响因子：
7.2
作者：
Pandey, Sona;Monshausen, Gabriele B.;Assmann, Sarah M.
通讯作者：
Assmann, Sarah M.