Arabidopsis heterotrimeric G-proteins play a critical role in host and nonhost resistance against Pseudomonas syringae pathogens.
Arabidopsis heterotrimeric G-proteins play a critical role in host and nonhost resistance against Pseudomonas syringae pathogens.
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DOI：
10.1371/journal.pone.0082445
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Mysore KS
中科院分区：
文献类型：
--
作者：
Lee S;Rojas CM;Ishiga Y;Pandey S;Mysore KS
异源三聚体G蛋白已被认为参与植物抗病性的许多方面，但它们在介导非宿主抗病性中的确切作用还不清楚。我们利用拟南芥Gα、Gβ和Gγ亚基的敲除突变体来评估异源三聚体G蛋白的特定亚基在响应宿主和非宿主假单胞菌病原体中的作用。缺乏功能性Gα、Gβ和Gγ1Gγ2蛋白的植物表现出对宿主和非宿主病原体的响应的增强的细菌生长和疾病易感性。Gγ单亚基Gγ1、Gγ2和Gγ3的突变不改变细菌的抗病性。当比较宿主病原体与非宿主病原体时，观察到亚基使用的一些特异性。Gα和Gβ的过表达导致非宿主病原体P.而Gβ的过表达导致寄主病原菌烟草赤星病菌的细菌生长减少，而Gα的过表达则没有。maculicola，与野生型Col-0。此外，细菌病原菌对气孔开度的调节在Gα和Gβ突变体中被改变，而在任何单或双Gγ突变体中不被改变。总之，这些数据证实了异源三聚体G蛋白在植物先天免疫和气孔调节中的关键作用。
Heterotrimeric G-proteins have been proposed to be involved in many aspects of plant disease resistance but their precise role in mediating nonhost disease resistance is not well understood. We evaluated the roles of specific subunits of heterotrimeric G-proteins using knock-out mutants of Arabidopsis Gα, Gβ and Gγ subunits in response to host and nonhost Pseudomonas pathogens. Plants lacking functional Gα, Gβ and Gγ1Gγ2 proteins displayed enhanced bacterial growth and disease susceptibility in response to host and nonhost pathogens. Mutations of single Gγ subunits Gγ1, Gγ2 and Gγ3 did not alter bacterial disease resistance. Some specificity of subunit usage was observed when comparing host pathogen versus nonhost pathogen. Overexpression of both Gα and Gβ led to reduced bacterial multiplication of nonhost pathogen P. syringae pv. tabaci whereas overexpression of Gβ, but not of Gα, resulted in reduced bacterial growth of host pathogen P. syringae pv. maculicola, compared to wild-type Col-0. Moreover, the regulation of stomatal aperture by bacterial pathogens was altered in Gα and Gβ mutants but not in any of the single or double Gγ mutants. Taken together, these data substantiate the critical role of heterotrimeric G-proteins in plant innate immunity and stomatal modulation in response to P. syringae.
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