ROCK is involved in vimentin phosphorylation and rearrangement induced by dengue virus.
ROCK is involved in vimentin phosphorylation and rearrangement induced by dengue virus.
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DOI：
10.1007/s12013-013-9665-x
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发表时间：
2013
影响因子：
2.6
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Lei, Shun;Tian, Yan-Ping;Xiao, Wei-Dong;Li, Shu;Rao, Xian-Cai;Zhang, Jun-Lei;Yang, Jie;Hu, Xiao-Mei;Chen, Wei
我们以前的研究表明，登革病毒2型（DENV 2）感染诱导波形蛋白重排成致密的结构在核周区。然而，这一现象的基本机制的特点很差。在目前的工作中，我们发现波形蛋白和Ser 71磷酸化波形蛋白在DENV 2感染的细胞中显示相似的分布。随着DENV 2感染的进展，DENV 2感染还诱导ROCK活化和波形蛋白在Ser 71处的磷酸化。此外，由DENV 2感染诱导的Ser 71磷酸化和波形蛋白重排被ROCK抑制剂Y-27632阻断。此外，DENV 2导致宿主细胞核周区域的内质网（ER）重新分布，这被Y-27632预处理部分阻断。总之，这些数据支持表明ROCK可能在DENV 2感染期间在控制调节波形蛋白和ER重排中起作用。我们假设，DENV 2感染，通过ROCK激活，诱导波形蛋白重排和ER重新分布周围的核周区域，这可能在锚定DENV 2复制位点的结构中发挥作用。
Our previous study showed that dengue virus 2 (DENV2) infection induces rearrangement of vimentin into dense structures at the perinuclear area. However, the underlying mechanism of this phenomenon is poorly characterized. In the present work, we found that vimentin and Ser71 phosphorylated vimentin display similar distributions in DENV2-infected cells. DENV2 infection also induced ROCK activation and phosphorylation of vimentin at Ser71 as the DENV2 infection progressed. Furthermore, Ser71 phosphorylation and vimentin rearrangement induced by DENV2 infection were blocked by the ROCK inhibitor Y-27632. In addition, DENV2 led to endoplasmic reticulum (ER) redistribution in the perinuclear region of the host cells, which was partially blocked by pretreatment with Y-27632. Together, these data support indicate that ROCK may have a role in governing regulating vimentin and ER rearrangement during DENV2 infection. We hypothesize that DENV2 infection, via ROCK activation, induces both vimentin rearrangement and ER redistribution around the perinuclear region, which may play a structural role in anchoring DENV2 to replication sites.
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影响因子：
30.3
作者：
Welsch S;Miller S;Romero-Brey I;Merz A;Bleck CK;Walther P;Fuller SD;Antony C;Krijnse-Locker J;Bartenschlager R
通讯作者：
Bartenschlager R
影响因子：
5.4
作者：
Karczewski, MK;Strebel, K
通讯作者：
Strebel, K
影响因子：
5.3
作者：
Sin, WC;Chen, XQ;Lim, L
通讯作者：
Lim, L
影响因子：
5.4
作者：
DEFER, C;BELIN, MT;BOULANGER, P
通讯作者：
BOULANGER, P
影响因子：
2.7
作者：
KATSUMOTO, T;MITSUSHIMA, A;KURIMURA, T
通讯作者：
KURIMURA, T