Inhibition of endogenous hydrogen sulfide production improves viral elimination in CVB3-infected myocardium in mice
Inhibition of endogenous hydrogen sulfide production improves viral elimination in CVB3-infected myocardium in mice
复制标题
抑制内源性硫化氢产生可改善小鼠 CVB3 感染心肌中的病毒消除
DOI：
10.1038/s41390-019-0281-3
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发表时间：
2019-01
影响因子：
3.6
通讯作者：
Gong Fangqi
中科院分区：
文献类型：
--
作者：
Hua Wang;Zheng Fenglei;Wang Yujia;Wang Ying;Fu Songling;Wang Wei;Xie Chunhong;Zhang Yiying;Gong Fangqi
背景：研究硫化氢是否与柯萨奇病毒B3 （CVB3）的复制和传播有相互作用。方法6周龄近交系雄性Balb/c小鼠腹腔注射CVB3。将小鼠随机分为4组（每组n= 10）： n组（假感染+载药）、C组（病毒+载药）、P组（病毒+dl-丙炔甘氨酸（PAG））和S组（病毒+硫化氢钠（NaHS））。每日腹腔注射PAG和NaHS，病毒接种后第4天杀死小鼠。取血清标本检测肿瘤坏死因子-α (TNFα)水平，取心脏标本进行组织学检查、50%组织培养感染剂量（TCID50）测定、逆转录-聚合酶链反应和Western blot分析。结果感染组间心重与体重之比及炎症评分差异无统计学意义。P组循环和局部TNFα、一氧化氮合酶2信使RNA和蛋白质浓度高于c组，S组低于c组，PAG和NaHS处理小鼠的病毒存量分别显著低于和高于仅接种CVB3的小鼠。结论抑制内源性硫化氢的产生有助于cvb3诱导的心肌炎急性病毒血症的病毒清除。
BackgroundTo find whether administration of hydrogen sulfide has interaction with coxsackie virus B3 (CVB3) replication and spread.MethodsSix-week-old inbred male Balb/c mice were injected intraperitoneally with CVB3. Mice were randomized to four groups (n= 10 for each group): group N (sham infection + vehicle), group C (virus + vehicle), group P (virus +dl-proparglygylcine (PAG)), and group S (virus + sodium hydrogen sulfide (NaHS)). PAG and NaHS were administered intraperitoneally daily and mice were killed on day 4 after viral inoculation. Serum specimens were obtained to assay tumor necrosis factor-α (TNFα) level, and heart specimens were harvested for histological examination, 50% tissue culture infection dose (TCID50) assay, reverse transcription-polymerase chain reaction and Western blot analysis.ResultsThe ratio of heart-weight to body-weight and inflammatory scores showed no significant difference between infected groups. The circulatory and local concentrations of TNFα, nitric oxide synthase 2 messenger RNA, and protein were higher in group P, and were lower in group S compared to those in group C. Mice treated with PAG and NaHS had significantly lower and higher viral stocks than those inoculated with CVB3 only, respectively.ConclusionInhibition of endogenous hydrogen sulfide production contributed to viral clearance in acute viremia of CVB3-induced myocarditis.
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影响因子：
7.4
作者：
Oh, Gi-Su;Pae, Hyun-Ock;Chung, Hun-Taeg
通讯作者：
Chung, Hun-Taeg
影响因子：
7
作者：
Burguera, E. F.;Vela-Anero, A.;Blanco, F. J.
通讯作者：
Blanco, F. J.
DOI：
10.1073/pnas.0400518101
发表时间：
2004-08
影响因子：
11.1
作者：
E. Padalko;T. Ohnishi;K. Matsushita;H. Sun;K. Fox‐Talbot;C. Bao;W. Baldwin;C. Lowenstein
通讯作者：
E. Padalko;T. Ohnishi;K. Matsushita;H. Sun;K. Fox‐Talbot;C. Bao;W. Baldwin;C. Lowenstein
影响因子：
5.6
作者：
Mirandola, Prisco;Gobbi, Giuliana;Vitale, Marco
通讯作者：
Vitale, Marco
影响因子：
5
作者：
Tanaka, T;Kanda, T;Kurabayashi, M
通讯作者：
Kurabayashi, M