A non-classical monocyte-derived macrophage subset provides a splenic replication niche for intracellular Salmonella.
A non-classical monocyte-derived macrophage subset provides a splenic replication niche for intracellular Salmonella.
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一个非经典的单核细胞来源的巨噬细胞亚群为细胞内沙门氏菌提供了一个脾复制利基。
DOI：
10.1016/j.immuni.2021.10.015
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发表时间：
2021-12-14
期刊：
影响因子：
32.4
通讯作者：
Avraham R
中科院分区：
文献类型：
--
作者：
Hoffman D;Tevet Y;Trzebanski S;Rosenberg G;Vainman L;Solomon A;Hen-Avivi S;Ben-Moshe NB;Avraham R
细胞内细菌和单核巨噬细胞之间的相互作用导致不同的细胞表型，这些表型可能决定感染的结果。单细胞RNA测序(scRNA-seq)的最新进展已经在单核群体中发现了多个亚群，但对它们在感染过程中的功能的影响是有限的。在这里，我们调查了细胞内鼠伤寒沙门氏菌(S.Tm)在早期全身感染期间的单核生态位。我们描述了脾中的月食样生长动力学，第一阶段的细菌控制是由组织驻留的红髓巨噬细胞介导的。第二阶段涉及以CD9表达为特征的巨噬细胞内广泛的细菌复制。我们证明了CD9+巨噬细胞诱导氧化脂质解毒的途径，这可能被细胞内的S.Tm所利用。我们证实CD9+巨噬细胞来源于非经典单核细胞(NCM)，NCM耗竭的小鼠对S.Tm感染具有更强的抵抗力。我们的研究定义了巨噬细胞亚群特异的宿主-病原体相互作用，这些相互作用决定了整个有机体的早期感染动力学和感染结果。在早期阶段，沙门氏菌动力学遵循类似食的动力学，CD9 MACs是沙门氏菌在月食期间的细胞内复制生态位CD9 MACs来源于非经典单核细胞，并诱导途径来解毒oxLDL CD9 MACs耗尽减少沙门氏菌感染和延长小鼠生存时间巨噬细胞是一个被广泛研究的具有多种功能的异质性群体，但目前不同的巨噬细胞亚群在细胞内感染中的作用有限。霍夫曼等人。揭示在小鼠感染早期，NCM产生CD9巨噬细胞，为沙门氏菌提供细胞内复制利基。
Interactions between intracellular bacteria and mononuclear phagocytes give rise to diverse cellular phenotypes that may determine the outcome of infection. Recent advances in single-cell RNA sequencing (scRNA-seq) have identified multiple subsets within the mononuclear population, but implications to their function during infection are limited. Here, we surveyed the mononuclear niche of intracellular Salmonella Typhimurium (S.Tm) during early systemic infection in mice. We described eclipse-like growth kinetics in the spleen, with a first phase of bacterial control mediated by tissue-resident red-pulp macrophages. A second phase involved extensive bacterial replication within a macrophage population characterized by CD9 expression. We demonstrated that CD9+ macrophages induced pathways for detoxificating oxidized lipids, that may be utilized by intracellular S.Tm. We established that CD9+ macrophages originated from non-classical monocytes (NCM), and NCM-depleted mice were more resistant to S.Tm infection. Our study defines macrophage subset-specific host-pathogen interactions that determine early infection dynamics and infection outcome of the entire organism. At early stages, Salmonella kinetics follows an eclipse-like dynamics CD9 Macs are an intracellular replication niche for Salmonella during eclipse CD9 Macs derive from non-classical monocytes and induce pathways to detoxify oxLDL CD9 Macs depletion reduces Salmonella infection and prolongs mice survival Macrophages are a well-studied heterogeneous population with a variety of functions, but the role of the different macrophage subsets during intracellular infection is presently limited. Hoffman et al. reveal that during early mice infection NCM give rise to CD9 macrophages that provide an intracellular replication niche for Salmonella.
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DOI：
10.1016/j.bbi.2018.09.004
发表时间：
2018-11
期刊：
Brain, behavior, and immunity
影响因子：
--
作者：
Ağaç D;Estrada LD;Maples R;Hooper LV;Farrar JD
通讯作者：
Farrar JD
DOI：
10.1084/jem.139.5.1189
发表时间：
1974-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Carter PB;Collins FM
通讯作者：
Collins FM
影响因子：
--
作者：
Bruehl, Hilke;Cihak, Josef;Mack, Matthias
通讯作者：
Mack, Matthias
影响因子：
2.9
作者：
De Jesus, Magdia;Park, Chae Gyu;Casadevall, Arturo
通讯作者：
Casadevall, Arturo
DOI：
10.1084/jem.20172020
发表时间：
2018-04-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Huang L;Nazarova EV;Tan S;Liu Y;Russell DG
通讯作者：
Russell DG