Induction of fibroblast growth factor-2 by electrical stimulation in cultured retinal Mueller cells
Induction of fibroblast growth factor-2 by electrical stimulation in cultured retinal Mueller cells
复制标题
电刺激培养的视网膜 Mueller 细胞诱导成纤维细胞生长因子 2
作者：
Tatsuhiko Sato;Tong;Fumihiko Takamatsu;T. Fujikado
已经证明，视网膜Mueller细胞（主要的神经胶质细胞）产生包括碱性成纤维细胞生长因子（FGF-2）的神经营养因子，并且电刺激增强FGF-2在中枢神经系统中的转录。本研究通过定量实时聚合酶链反应和蛋白质印迹法研究了电刺激对培养的大鼠Mueller细胞中FGF-2诱导的影响。与对照组相比，电刺激后FGF-2的信使RNA和蛋白质均显著上调。这些结果表明，电刺激可以直接诱导视网膜中FGF-2的产生。
It has been demonstrated that retinal Mueller cells, the predominant glial cells, produce neurotrophic factors including basic fibroblast growth factor (FGF-2), and that electrical stimulation enhances the transcription of FGF-2 in the central nervous system. In this study, the effect of electrical stimulation on the induction of FGF-2 in cultured rat Mueller cells was investigated by quantitative real-time polymerase chain reaction and western blotting. Both the messenger RNA and protein of FGF-2 were significantly upregulated after electrical stimulation compared with that of controls. These results suggest that electrical stimulation may directly induce the production of FGF-2 in the retina.
影响因子：
3.4
作者：
Al-Majed, AA;Brushart, TM;Gordon, T
通讯作者：
Gordon, T
Muller 细胞中碱性成纤维细胞生长因子诱导碱性成纤维细胞生长因子 mRNA。
影响因子：
4.4
作者：
Cao,W;Wen,R;Li,F;Cheng,T;Steinberg,RH
通讯作者：
Steinberg,RH