A Lipid Transfer Protein Increases the Glutathione Content and Enhances Arabidopsis Resistance to a Trichothecene Mycotoxin.
A Lipid Transfer Protein Increases the Glutathione Content and Enhances Arabidopsis Resistance to a Trichothecene Mycotoxin.
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DOI：
10.1371/journal.pone.0130204
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tumer NE
中科院分区：
文献类型：
--
作者：
McLaughlin JE;Bin-Umer MA;Widiez T;Finn D;McCormick S;Tumer NE
赤霉病（Fusarium head blight，FHB）是世界范围内最重要的植物病害之一，影响小麦、大麦和其他小谷物。单端孢霉烯类真菌毒素如脱氧雪腐镰刀菌烯醇（DON）在谷物中积累，对人类和动物构成食品安全风险和健康危害。尽管进行了大量的育种工作，但仍没有高抗性的小麦或大麦品种。我们筛选了活化标记的拟南芥种群对拟南芥素（Tcin）的抗性，所述拟南芥素是与DON相同类别的B型拟南芥烯。在这里，我们发现，一个耐药株系，抗二孢噻吩1（trr 1）含有一个T-DNA插入上游的两个非特异性脂质转移蛋白（nsLTP）基因，AtLTP4.4和AtLTP4.5。两个nsLTP基因的表达诱导trr 1超过10倍，相对于野生型。相对于野生型或载体转化系，AtLTP4.4的过表达在拟南芥和酿酒酵母中提供了比AtLTP4.5更大的Tcin抗性，表明了保守的保护机制。Tcin处理增加了拟南芥中活性氧（ROS）的产生，ROS的染色与叶绿体、细胞壁和质外体有关。相对于对照，拟南芥和过表达AtLTP4.4的酵母中的ROS水平减弱。外源添加谷胱甘肽和其他抗氧化剂增强了拟南芥对Tcin的抗性，而添加谷胱甘肽合成抑制剂丁硫基亚砜亚胺增加了敏感性，表明抗性是由谷胱甘肽介导的。总谷胱甘肽含量显着较高，在拟南芥和酵母过表达AtLTP4.4相对于控制，突出AtLTP4.4在维持氧化还原状态的重要性。这些结果表明，倍半萜类化合物引起ROS积累，AtLTP4.4的过表达通过增加基于谷胱甘肽的抗氧化防御来保护免受倍半萜类化合物诱导的氧化应激。
Fusarium head blight (FHB) or scab is one of the most important plant diseases worldwide, affecting wheat, barley and other small grains. Trichothecene mycotoxins such as deoxynivalenol (DON) accumulate in the grain, presenting a food safety risk and health hazard to humans and animals. Despite considerable breeding efforts, highly resistant wheat or barley cultivars are not available. We screened an activation tagged Arabidopsis thaliana population for resistance to trichothecin (Tcin), a type B trichothecene in the same class as DON. Here we show that one of the resistant lines identified, trichothecene resistant 1 (trr1) contains a T-DNA insertion upstream of two nonspecific lipid transfer protein (nsLTP) genes, AtLTP4.4 and AtLTP4.5. Expression of both nsLTP genes was induced in trr1 over 10-fold relative to wild type. Overexpression of AtLTP4.4 provided greater resistance to Tcin than AtLTP4.5 in Arabidopsis thaliana and in Saccharomyces cerevisiae relative to wild type or vector transformed lines, suggesting a conserved protection mechanism. Tcin treatment increased reactive oxygen species (ROS) production in Arabidopsis and ROS stain was associated with the chloroplast, the cell wall and the apoplast. ROS levels were attenuated in Arabidopsis and in yeast overexpressing AtLTP4.4 relative to the controls. Exogenous addition of glutathione and other antioxidants enhanced resistance of Arabidopsis to Tcin while the addition of buthionine sulfoximine, an inhibitor of glutathione synthesis, increased sensitivity, suggesting that resistance was mediated by glutathione. Total glutathione content was significantly higher in Arabidopsis and in yeast overexpressing AtLTP4.4 relative to the controls, highlighting the importance of AtLTP4.4 in maintaining the redox state. These results demonstrate that trichothecenes cause ROS accumulation and overexpression of AtLTP4.4 protects against trichothecene-induced oxidative stress by increasing the glutathione-based antioxidant defense.
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影响因子：
4.9
作者：
Desmond, Olivia J.;Manners, John M.;Kazan, Kemal
通讯作者：
Kazan, Kemal
影响因子：
6.9
作者：
De Coninck, Barbara;Carron, Delphine;Cammue, Bruno P. A.
通讯作者：
Cammue, Bruno P. A.
DOI：
10.1073/pnas.78.1.238
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FRIED, HM;WARNER, JR
通讯作者：
WARNER, JR
影响因子：
4.2
作者：
Ansari, Khairul I.;Doyle, Siamsa M.;Doohan, Fiona M.
通讯作者：
Doohan, Fiona M.
影响因子：
4.2
作者：
Audenaert K;Vanheule A;Höfte M;Haesaert G
通讯作者：
Haesaert G