Long-distance control of potato storage organ formation by SELF PRUNING 3D and FLOWERING LOCUS T-like 1.
Long-distance control of potato storage organ formation by SELF PRUNING 3D and FLOWERING LOCUS T-like 1.
复制标题
利用SELF PRUNING 3D和FLOWERLOCUS T-like 1远程控制马铃薯贮藏器官的形成。
DOI：
10.1016/j.xplc.2023.100547
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发表时间：
2023-05-08
影响因子：
10.5
通讯作者：
Song, Botao
中科院分区：
文献类型：
--
作者：
Jing, Shenglin;Jiang, Peng;Sun, Xiaomeng;Yu, Liu;Wang, Enshuang;Qin, Jun;Zhang, Fei;Prat, Salome;Song, Botao
关键词：
植物程序分生组织相关的发展开关及时适应不断变化的环境。马铃薯（Solanum tuberosum L.）块茎通过其末端的放射状扩张而与专门化的地下分枝或匍匐茎区别开来。在这个过程中，匍匐茎顶端和最接近的腋芽进入休眠状态，导致块茎的眼睛，这是重新激活第二年春天，并产生克隆相同的植物。马铃薯FLOWESTERN LOCUS T同源物SELF-PRUNING 6A（StSP 6A）先前被鉴定为主要的块茎诱导信号，其整合了日长线索以控制储存开关。然而，是否其他一些远程信号也作为块茎器官发生刺激仍然未知。在这里，我们表明，成花素自我修剪3D（StSP 3D）和FLOWLOCUS T样1（StFTL 1）基因被短日照激活，类似于StSP 6A。StSP 3D或StFTL 1的过表达促进块茎形成下非诱导长的日子，这些蛋白质的块茎诱导活性是嫁接传递。使用非块茎轴承野生种马铃薯，一个自然的SP 6A无效突变体，我们表明，叶表达的SP 6A的StSP 3D的远程活动是不确定的。StSP 3D和StFTL 1介导StSP 6A在匍匐茎顶端的二次激活，导致该tuberigen信号放大。观察到StSP 3D和StFTL 1与StSP 6A结合相同的蛋白质伴侣，表明它们也可以形成转录活性复合物。总之，我们的研究结果表明，额外的移动的块茎诱导信号的光周期途径进行调节。块茎诱导与开花调控途径具有共同的调控元件。这项工作报告说，StSP 3D和StFTL 1，像StSP 6A，是移动的蛋白，从叶片运输到匍匐茎尖的块茎诱导，SP 3D可以诱导块茎形成，即使在没有叶表达的SeSP 6A。
Plants program their meristem-associated developmental switches for timely adaptation to a changing environment. Potato (Solanum tuberosum L.) tubers differentiate from specialized belowground branches or stolons through radial expansion of their terminal ends. During this process, the stolon apex and closest axillary buds enter a dormancy state that leads to tuber eyes, which are reactivated the following spring and generate a clonally identical plant. The potato FLOWERING LOCUS T homolog SELF-PRUNING 6A (StSP6A) was previously identified as the major tuber-inducing signal that integrates day-length cues to control the storage switch. However, whether some other long-range signals also act as tuber organogenesis stimuli remains unknown. Here, we show that the florigen SELF PRUNING 3D (StSP3D) and FLOWERING LOCUS T-like 1 (StFTL1) genes are activated by short days, analogously to StSP6A. Overexpression of StSP3D or StFTL1 promotes tuber formation under non-inductive long days, and the tuber-inducing activity of these proteins is graft transmissible. Using the non-tuber-bearing wild species Solanum etuberosum, a natural SP6A null mutant, we show that leaf-expressed SP6A is dispensable for StSP3D long-range activity. StSP3D and StFTL1 mediate secondary activation of StSP6A in stolon tips, leading to amplification of this tuberigen signal. StSP3D and StFTL1 were observed to bind the same protein partners as StSP6A, suggesting that they can also form transcriptionally active complexes. Together, our findings show that additional mobile tuber-inducing signals are regulated by the photoperiodic pathway. Tuber induction shares common regulatory elements with flowering regulatory pathways. This work reports that StSP3D and StFTL1, like StSP6A, are mobile proteins that are transported from leaves to stolon tips for tuber induction, and SP3D can induce tuber formation even in the absence of leaf-expressed SeSP6A.
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影响因子：
7.2
作者：
Lee, Jungeun;Oh, Mijin;Lee, Ilha
通讯作者：
Lee, Ilha
影响因子：
64.5
作者：
Hendelman, Anat;Zebell, Sophia;Lippman, Zachary B.
通讯作者：
Lippman, Zachary B.
影响因子：
64.8
作者：
Kloosterman, Bjorn;Abelenda, Jose A.;Bachem, Christian W. B.
通讯作者：
Bachem, Christian W. B.
影响因子：
9.4
作者：
KUMAR, D;WAREING, PF
通讯作者：
WAREING, PF
影响因子：
3
作者：
GREGORY, LE
通讯作者：
GREGORY, LE