Application of carbodiimide derivatized synovial fluid to enhance extrasynovial tendon gliding ability.
Application of carbodiimide derivatized synovial fluid to enhance extrasynovial tendon gliding ability.
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DOI：
10.1016/j.jhsa.2010.12.015
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Zhao C
中科院分区：
文献类型：
--
作者：
Ikeda J;Sun YL;An KN;Amadio PC;Zhao C
研究用碳二亚胺衍生的滑液（SF）对滑膜外肌腱进行表面改性对滑膜外肌腱滑动能力的影响，以用于可能的肌腱移植应用。我们使用了63条犬后腿的腓骨长肌腱。我们立即使用扫描电子显微镜（SEM）对3个肌腱进行形态学评估;这些肌腱作为正常肌腱组。另外60个肌腱被随机分配到6个实验组中的每一个，所述实验组用（1）对照（盐水）;（2）1% 1-乙基-3-甲基-N-（2-甲基-N-苯基）-N-（2-甲基（3-二甲基氨基丙基）碳二亚胺盐酸盐（EDC）+1%N-羟基琥珀酰亚胺（NHS）（仅cd）;（3）1%EDC/NHS +10%明胶（cd-G）;（4）SF +1%EDC/NHS +10%明胶（cd-SF-G）;（5）仅SF;（6）SF +1%EDC/NHS（cd-SF）。我们测量了1,000次模拟屈伸运动循环的滑动阻力。扫描电镜观察肌腱表面光滑度。与每组中的第一个周期相比，在对照组、仅cd组、cd-明胶组、仅SF组和cd-SF组中，肌腱运动1,000个周期后的滑动阻力显著增加（p<0.05）。相比之下，我们发现cd-SF-G治疗组的第一个周期和1,000个周期之间的滑动阻力没有显著差异。此外，在1,000次肌腱运动循环后，cd-SF、cd-G和cd-SF-G组的滑动阻力显著低于对照组（p<0.05），并且cd-SF-G组的滑动阻力显著低于cd-G和cd-SF组（p<0.05）。在SEM上，用cd-SF-G处理的表面在1，000次循环后是光滑的，而其它表面是粗糙的。用cd-SF-G对滑膜外肌腱进行表面改性以改善肌腱的滑动能力。这种治疗方法在临床上可能有助于改善肌腱自体移植的结果。
To investigate the effects of surface modification of extrasynovial tendon with a carbodiimide derivatized synovial fluid (SF) on the gliding ability of extrasynovial tendon for a possible tendon graft application. We used 63 peroneus longus tendons from canine hind legs. We immediately assessed 3 tendons morphologically using a scanning electron microscope (SEM); these served as the normal tendon group. The other 60 tendons were randomly assigned to each of 6 experimental groups treated with (1) control (saline); (2) 1% 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) plus 1% N-hydroxysuccinimide (NHS) (cd only); (3) 1% EDC/NHS plus 10% gelatin (cd-G); (4) SF plus 1% EDC/NHS plus 10% gelatin (cd-SF-G); (5) SF only; or (6) SF plus 1% EDC/NHS (cd-SF). We measured the gliding resistance for 1,000 cycles of simulated flexion-extension motion. We also observed the tendon surface smoothness by SEM. Compared with the first cycle in each group, the gliding resistance after 1,000 cycles of tendon motion was significantly increased in the control, cd only, cd-gelatin, SF only, and cd-SF groups (p<.05). In contrast, we found no significant difference in gliding resistance between the first cycle and 1,000 cycles for the cd-SF-G–treated group. In addition, the gliding resistance in the cd-SF, cd-G, and cd-SF-G groups was significantly lower than the control group after 1,000 cycles of tendon motion (p<.05) and the gliding resistance of the cd-SF-G group was significantly lower than both the cd-G and cd-SF groups (p<.05). On SEM, the surface treated with cd-SF-G was smooth after 1,000 cycles, whereas the other surfaces were rough. Surface modification of extrasynovial tendon with cd-SF-G improves tendon gliding ability. This treatment may be useful clinically in improving the outcomes of tendon autografts.
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影响因子：
2.9
作者：
JAY, GD
通讯作者：
JAY, GD
影响因子：
27.4
作者：
RADIN, EL;PAUL, IL;SCHOTTSTAEDT, ES
通讯作者：
SCHOTTSTAEDT, ES
影响因子：
1.9
作者：
CARLSON, GD;BOTTE, MJ;WOO, SLY
通讯作者：
WOO, SLY
影响因子：
1.9
作者：
AMADIO, PC;WOOD, MB;BOGARD, SD
通讯作者：
BOGARD, SD
DOI：
10.1016/s0266-7681(05)80113-5
发表时间：
1995-10-01
期刊：
JOURNAL OF HAND SURGERY-BRITISH AND EUROPEAN VOLUME
影响因子：
--
作者：
COERT, JH;UCHIYAMA, S;AN, KN
通讯作者：
AN, KN