Periventricular Demyelination and Axonal Pathology Is Associated with Subependymal Virus Spread in a Murine Model for Multiple Sclerosis
Periventricular Demyelination and Axonal Pathology Is Associated with Subependymal Virus Spread in a Murine Model for Multiple Sclerosis
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脑室周围脱髓鞘和轴突病理与多发性硬化症小鼠模型中室管膜下病毒传播相关
DOI：
10.1159/000336563
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发表时间：
2012
期刊：
影响因子：
4.6
通讯作者：
A. Beineke
中科院分区：
文献类型：
--
作者：
Kummerfeld M;Seehusen F;Klein S;Ulrich R;Kreutzer R;Gerhauser I;Herder V;Baumgärtner W;A. Beineke
目的Theiler氏鼠脑脊髓炎病毒（TMEV）感染小鼠是一种广泛应用的脱髓鞘疾病，如多发性硬化症（MS）的动物模型。本研究的目的是确定TMEV的传播和脱髓鞘在实验感染的易感SJL/J小鼠和耐药C57 BL/6小鼠的脑中的地形差异。方法脱髓鞘通过Luxol坚牢蓝和甲酚紫染色和轴突损伤通过神经胶质特异性和β-淀粉样前体蛋白特异性免疫组织化学证实。通过免疫组织化学和原位杂交定量分析中枢神经系统（CNS）内的病毒传播。此外，感染细胞的表型通过共聚焦激光扫描microscopic. ResultsA早期短暂的脑室周围细胞的感染，然后脱髓鞘和轴突病变周围的第四脑室在SJL/J小鼠注意到。脑室周围和脑干脱髓鞘与小胶质细胞/巨噬细胞和少突胶质细胞的主要感染有关。ConclusionsSummarized，室管膜感染和subjunctions传播到脑实质以及区域病毒清除的演示，尽管正在进行脱髓鞘和轴突损伤在其他CNS舱室允许TME发病机制的新见解。TMEV CNS相互作用的这一新方面将增强对该MS模型中脑损伤和再生能力的区域特异性易感性的理解。
ObjectivesTheiler’s murine encephalomyelitis virus (TMEV) infection of mice is a widely used animal model for demyelinating disorders, such as multiple sclerosis (MS). The aim of the present study was to identify topographical differences of TMEV spread and demyelination in the brain of experimentally infected susceptible SJL/J mice and resistant C57BL/6 mice.MethodsDemyelination was confirmed by Luxol fast blue and cresyl violet staining and axonal damage by neurofilament-specific and β-amyloid precursor protein-specific immunohistochemistry. Viral dissemination within the central nervous system (CNS) was quantified by immunohistochemistry and in situ hybridization. Further, the phenotype of infected cells was determined by confocal laser scanning microscopy.ResultsAn early transient infection of periventricular cells followed by demyelination and axonopathies around the fourth ventricle in SJL/J mice was noticed. Periventricular and brain stem demyelination was associated with a predominant infection of microglia/macrophages and oligodendrocytes.ConclusionsSummarized, the demonstration of ependymal infection and subjacent spread into the brain parenchyma as well as regional virus clearance despite ongoing demyelination and axonal damage in other CNS compartments allows new insights into TME pathogenesis. This novel aspect of TMEV CNS interaction will enhance the understanding of region-specific susceptibilities to injury and regenerative capacities of the brain in this MS model.
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影响因子：
5.3
作者：
Kreutzer R;Kreutzer M;Pröpsting MJ;Sewell AC;Leeb T;Naim HY;Baumgärtner W
通讯作者：
Baumgärtner W
影响因子：
12.7
作者：
Gröters, S;Alldinger, S;Baumgärtner, W
通讯作者：
Baumgärtner, W
影响因子：
6
作者：
Brück, W
通讯作者：
Brück, W
影响因子：
--
作者：
Boster, Aaron;Hreha, Stephanie;Khan, Omar
通讯作者：
Khan, Omar
白质病变的成像。
影响因子：
1.9
作者：
S. Kanekar
通讯作者：
S. Kanekar