Using metabarcoding to compare the suitability of two blood-feeding leech species for sampling mammalian diversity in North Borneo.
Using metabarcoding to compare the suitability of two blood-feeding leech species for sampling mammalian diversity in North Borneo.
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DOI：
10.1111/1755-0998.12943
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发表时间：
2019-01
影响因子：
7.7
通讯作者：
Rossiter SJ
中科院分区：
文献类型：
--
作者：
Drinkwater R;Schnell IB;Bohmann K;Bernard H;Veron G;Clare E;Gilbert MTP;Rossiter SJ
应用高通量测序（HTS）对混合样品进行元条形码编码为保护生物学提供了新的机会。最近，从水蛭肠道中成功检测到猎物DNA，这提高了水蛭和其他吸血无脊椎动物可能作为哺乳动物有用的采样器的可能性。然而，很少有人知道是否同域水蛭物种不同，在他们的饮食偏好，以及这是否有一个关系到他们的相对适合监测当地哺乳动物的多样性。为了解决这些问题，我们收集了空间匹配的两个同属水蛭物种Haemadipsa picta和Haemadipsa sumatrana在婆罗洲的低地雨林的样本。对于每个物种，我们将约500只水蛭分成10只一批，进行PCR以靶向哺乳动物16S rRNA基因座的一部分，并使用Illumina MiSeq对扩增子文库进行测序。总之，我们确定了14个哺乳动物属，跨越9个家庭和5个订单的序列。我们发现更多的检测，和更高的OTU的多样性，在H. picta相比，H. sumatrana，啮齿动物只存在于前水蛭物种。然而，对整个景观的样本进行比较发现，水蛭物种之间的哺乳动物群落组成没有显着差异。因此，我们建议，H. picta是更合适的iDNA采样器在这个退化的婆罗洲森林。我们的结论是，无脊椎动物采样器的选择可以影响不同哺乳动物群体的可检测性，这在设计iDNA研究时应该考虑在内。
The application of high‐throughput sequencing (HTS) for metabarcoding of mixed samples offers new opportunities in conservation biology. Recently, the successful detection of prey DNA from the guts of leeches has raised the possibility that these, and other blood‐feeding invertebrates, might serve as useful samplers of mammals. Yet little is known about whether sympatric leech species differ in their feeding preferences, and whether this has a bearing on their relative suitability for monitoring local mammalian diversity. To address these questions, we collected spatially matched samples of two congeneric leech species Haemadipsa picta and Haemadipsa sumatrana from lowland rainforest in Borneo. For each species, we pooled ~500 leeches into batches of 10 individuals, performed PCR to target a section of the mammalian 16S rRNA locus and undertook sequencing of amplicon libraries using an Illumina MiSeq. In total, we identified sequences from 14 mammalian genera, spanning nine families and five orders. We found greater numbers of detections, and higher diversity of OTUs, in H. picta compared with H. sumatrana, with rodents only present in the former leech species. However, comparison of samples from across the landscape revealed no significant difference in mammal community composition between the leech species. We therefore suggest that H. picta is the more suitable iDNA sampler in this degraded Bornean forest. We conclude that the choice of invertebrate sampler can influence the detectability of different mammal groups and that this should be accounted for when designing iDNA studies.
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影响因子：
4.9
作者：
Calvignac-Spencer, Sebastien;Merkel, Kevin;Leendertz, Fabian H.
通讯作者：
Leendertz, Fabian H.
影响因子：
--
作者：
Gomez, Andres;Kolokotronis, Sergios-Orestis
通讯作者：
Kolokotronis, Sergios-Orestis
影响因子：
1.7
作者：
Gannon, Wumm L.;Sikes, Robept S.
通讯作者：
Sikes, Robept S.
影响因子：
6.1
作者：
Chao, Anne;Gotelli, Nicholas J.;Ellison, Aaron M.
通讯作者：
Ellison, Aaron M.
DOI：
10.1098/rstb.2011.0049
发表时间：
2011-11-27
影响因子：
6.3
作者：
Ewers, Robert M.;Didham, Raphael K.;Turner, Edgar C.
通讯作者：
Turner, Edgar C.