Unique Glycoform-Dependent Monoclonal Antibodies for Mouse Mucin 21
Unique Glycoform-Dependent Monoclonal Antibodies for Mouse Mucin 21
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DOI：
10.3390/ijms23126718
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发表时间：
2022-06-16
影响因子：
5.6
通讯作者：
Irimura, Tatsuro
中科院分区：
文献类型：
--
作者：
Nishida, Jun;Shichino, Shigeyuki;Tsukui, Tatsuya;Hoshino, Mayumi;Okada, Tomoko;Okada, Kyoko;Yi, Yuri;Toraya-Brown, Seiko;Mochizuki, Miho;Koizumi, Ryouta;Ishii-Schrade, Katrin;Denda-Nagai, Kaori;Irimura, Tatsuro
粘蛋白21（Muc 21）/epiglycanin在鳞状上皮的顶端表面上表达，并且具有潜在的保护作用，这被认为与其独特的糖型相关，而其异常糖基化与一些癌的恶性行为有关。尽管糖型的重要性，我们缺乏工具来检测小鼠Muc 21的特定糖型。在这项研究中，我们产生了两个单克隆抗体（mAb），识别不同的糖型的Muc 21。我们使用表达Muc 21的TA 3-Ha细胞或用Muc 21转染的中国仓鼠卵巢（CHO）-K1细胞的膜裂解物作为抗原。利用Mu 21糖基化变体细胞的特异性测试显示，mAb 1A 4 -1识别携带以半乳糖残基终止的聚糖的Mu 21，而mAb 18 A11识别携带唾液酸化聚糖的Mu 21。mAb 1A 4 -1在体外和小鼠移植肿瘤中染色了大多数小鼠乳腺癌TA 3-Ha细胞，而mAb 18 A11仅识别这些细胞的亚群。mAb 1A 4 -1可用于免疫组化检测正常鳞状上皮中的Muc 21。总之，这些mAb识别由肽部分和O-聚糖的组合形成的不同Muc 21表位。
Mucin 21(Muc21)/epiglycanin is expressed on apical surfaces of squamous epithelia and has potentially protective roles, which are thought to be associated with its unique glycoforms, whereas its aberrant glycosylation is implicated in the malignant behaviors of some carcinomas. Despite the importance of glycoforms, we lack tools to detect specific glycoforms of mouse Muc21. In this study, we generated two monoclonal antibodies (mAbs) that recognize different glycoforms of Muc21. We used membrane lysates of Muc21-expressing TA3-Ha cells or Chinese hamster ovary (CHO)-K1 cells transfected with Muc21 as antigens. Specificity testing, utilizing Muc21 glycosylation variant cells, showed that mAb 1A4-1 recognized Muc21 carrying glycans terminated with galactose residues, whereas mAb 18A11 recognized Muc21 carrying sialylated glycans. mAb 1A4-1 stained a majority of mouse mammary carcinoma TA3-Ha cells in vitro and in engrafted tumors in mice, whereas mAb 18A11 recognized only a subpopulation of these. mAb 1A4-1 was useful in immunohistochemically detecting Muc21 in normal squamous epithelia. In conclusion, these mAbs recognize distinct Muc21 epitopes formed by combinations of peptide portions and O-glycans.
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影响因子：
7
作者：
Tian, Yuan;Denda-Nagai, Kaori;Tsukui, Tatsuya;Ishii-Schrade, Katrin B.;Okada, Kyoko;Nishizono, Yoshihiro;Matsuzaki, Kosuke;Hafley, Margarete;Bresalier, Robert S.;Irimura, Tatsuro
通讯作者：
Irimura, Tatsuro
影响因子：
4.3
作者：
Sorensen, AL;Reis, CA;Clausen, H
通讯作者：
Clausen, H
影响因子：
64.5
作者：
KINGSLEY, DM;KOZARSKY, KF;KRIEGER, M
通讯作者：
KRIEGER, M
影响因子：
4
作者：
Kamata, M;Denda-Nagai, K;Irimura, T
通讯作者：
Irimura, T
DOI：
10.1016/j.bbcan.2015.08.003
发表时间：
2015-12
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Chugh S;Gnanapragassam VS;Jain M;Rachagani S;Ponnusamy MP;Batra SK
通讯作者：
Batra SK