Different populations of GABAergic neurons in the visual cortex and hippocampus of cat contain somatostatin- or cholecystokinin- immunoreactive material
Different populations of GABAergic neurons in the visual cortex and hippocampus of cat contain somatostatin- or cholecystokinin- immunoreactive material
复制标题
猫视觉皮层和海马中不同的 GABA 能神经元群含有生长抑素或胆囊收缩素免疫反应物质
作者：
P. Somogyi;A. Hodgson;A. D. Smith;M. Nunzi;A. Gorio;J. Wu
在猫的海马和视觉皮层研究了γ -氨基丁酸（GABA）、谷氨酸脱羧酶（GAD）和胆囊收缩素（CCK）或生长抑素免疫反应物质在同一神经元中的共存。用无标记抗体酶法反应同一神经元的1微米厚的连续切片，以显示不同的抗原。所有皮层CCK和生长抑素免疫反应神经元，以及海马区所有CCK免疫反应神经元和大部分生长抑素免疫反应神经元，在连续切片中也对GABA免疫反应。在对广泛性焦虑症有免疫反应的神经元中，通常可以证明其中一种肽和GABA的免疫反应性。GABA抗血清显示，GABA免疫反应神经元存在于皮质和海马的所有层，其形状、大小和分布与GABA免疫反应神经元相似。所有gad免疫反应神经元也呈GABA阳性，但后者染色显示额外的神经元。CCK/GABA-和生长抑素/GABA免疫反应神经元主要分布在视觉皮层的第II层和上III层以及第V层和第VI层。CCK/GABA-免疫反应神经元最常见于海马的东层、锥体和分子层以及齿状回的多形细胞层。生长抑素/GABA免疫反应神经元主要分布于牙筋膜门区和牙筋膜层。这两种多肽不能在同一个神经元中找到。大多数具有GABA免疫反应的神经元没有对这两种肽进行染色。gaba能皮质神经元中CCK-和生长抑素免疫反应物质的存在，提高了神经活性肽影响gaba能神经传递的可能性。
The coexistence of gamma-aminobutyric acid (GABA), glutamate decarboxylase (GAD), and cholecystokinin (CCK)- or somatostatin- immunoreactive material in the same neurons was studied in the hippocampus and visual cortex of the cat. One-micrometer-thick serial sections of the same neuron were reacted to reveal different antigens by the unlabeled antibody enzyme method. All CCK- and somatostatin- immunoreactive neurons in the cortex and all CCK-immunoreactive and the majority of somatostatin-immunoreactive neurons in the hippocampus that could be examined in serial sections were also immunoreactive for GABA. In neurons that were immunoreactive for GAD it was often possible to demonstrate immunoreactivity for one of the peptides as well as for GABA. GABA-immunoreactive neurons, as revealed by an antiserum to GABA, were present in all layers of the cortex and hippocampus, and their shape, size, and distribution were similar to GAD-immunoreactive neurons. All GAD-immunoreactive neurons were also positive for GABA, but the latter staining revealed additional neurons. CCK/GABA- and somatostatin/GABA-immunoreactive neurons were present mainly in layers II and upper III and in layers V and VI in the visual cortex. CCK/GABA- immunoreactive neurons were most frequently present in the strata oriens, pyramidale, and moleculare of the hippocampus and in the polymorph cell layer of the dentate gyrus. Somatostatin/GABA- immunoreactive neurons were localized mainly in the stratum oriens and in the hilus of the fascia dentata. The two peptides could not be found in the same neuron. The majority of neurons that were GABA immunoreactive did not stain for either peptide. The presence of CCK- and somatostatin-immunoreactive material in GABAergic cortical neurons raises the possibility that neuroactive peptides affect GABAergic neurotransmission.
大鼠和猴子大脑皮层中的胆囊收缩素免疫反应性神经元形成对称突触，并与血管密切相关。
DOI：
10.1073/pnas.80.8.2400
发表时间：
1983
影响因子：
11.1
作者：
Hendry,SH;Jones,EG;Beinfeld,MC
通讯作者：
Beinfeld,MC
大鼠脑中原生长抑素特异性抗原：通过前生长抑素合成序列的抗血清进行免疫细胞化学染色定位。
DOI：
10.1073/pnas.80.9.2780
发表时间：
1983
影响因子：
11.1
作者：
Lechan,RM;Goodman,RH;Rosenblatt,M;Reichlin,S;Habener,JF
通讯作者：
Habener,JF
多种形式的免疫反应性生长抑素：大鼠神经组织和非神经组织以及门静脉血浆中分布的比较。
影响因子：
4.8
作者：
Patel,YC;Wheatley,T;Ning,C
通讯作者：
Ning,C