Transcriptomic and proteomic analysis reveals mechanisms of embryo abortion during chrysanthemum cross breeding.
Transcriptomic and proteomic analysis reveals mechanisms of embryo abortion during chrysanthemum cross breeding.
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转录组和蛋白质组分析揭示了菊花杂交育种过程中胚胎败育的机制
DOI：
10.1038/srep06536
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发表时间：
2014-10-07
影响因子：
4.6
通讯作者：
Teng N
中科院分区：
文献类型：
--
作者：
Zhang F;Wang Z;Dong W;Sun C;Wang H;Song A;He L;Fang W;Chen F;Teng N
胚败育是菊花杂交育种失败的主要原因，但与胚败育相关的基因和蛋白质却知之甚少。在这里，我们应用RNA测序和同量异序标签的相对和绝对定量（iTRAQ）分析正常和流产胚胎的转录组和蛋白质组学概况。从正常和流产胚胎中获得了超过68，000个注释的unigenes和700个蛋白质。功能分析表明，140个差异表达基因（DEG）和41个差异表达蛋白（DEPs）与胚胎败育有关。与细胞死亡，蛋白质降解，活性氧清除和应激反应转录因子相关的大多数DEG和DEP在流产胚胎中相对于正常胚胎显著上调。与此相反，与细胞分裂和扩展、细胞骨架、蛋白质合成和能量代谢相关的基因和蛋白质在败育胚胎中表达显著下调。此外，败育胚胎具有最高的活性的三个刽子手半胱天冬酶样酶。这些结果表明，胚胎败育可能与细胞程序性死亡有关，衰老或死亡相关基因或蛋白参与了胚胎败育。这增加了我们对胚胎败育的理解，并将有助于未来菊花和其他作物的杂交育种。
Embryo abortion is the main cause of failure in chrysanthemum cross breeding and the genes and proteins associated with embryo abortion are poorly understood. Here, we applied RNA sequencing and isobaric tags for relative and absolute quantitation (iTRAQ) to analyse transcriptomic and proteomic profiles of normal and abortive embryos. More than 68,000 annotated unigenes and 700 proteins were obtained from normal and abortive embryos. Functional analysis showed that 140 differentially expressed genes (DEGs) and 41 differentially expressed proteins (DEPs) were involved in embryo abortion. Most DEGs and DEPs associated with cell death, protein degradation, reactive oxygen species scavenging and stress-response transcriptional factors were significantly up-regulated in abortive embryos relative to normal embryos. In contrast, most genes and proteins related to cell division and expansion, the cytoskeleton, protein synthesis and energy metabolism were significantly down-regulated in abortive embryos. Furthermore, abortive embryos had the highest activity of three executioner caspase-like enzymes. These results indicate that embryo abortion may be related to programmed cell death and the senescence- or death-associated genes or proteins contribute to embryo abortion. This adds to our understanding of embryo abortion and will aid in the cross breeding of chrysanthemum and other crops in the future.
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影响因子：
11.8
作者：
Kradolfer, David;Wolff, Philip;Kohler, Claudia
通讯作者：
Kohler, Claudia
影响因子：
13.8
作者：
Chen, Xiaoping;Zhu, Wei;Liang, Xuanqiang
通讯作者：
Liang, Xuanqiang
影响因子：
9.4
作者：
Guggisberg, Alessia;Lai, Zhao;Rieseberg, Loren H.
通讯作者：
Rieseberg, Loren H.
影响因子：
11.6
作者：
Kim, Dong Hwan;Yamaguchi, Shinjiro;Choi, Giltsu
通讯作者：
Choi, Giltsu
DOI：
10.1016/j.bbagrm.2011.04.004
发表时间：
2011-08-01
影响因子：
4.7
作者：
Kohler, Claudia;Kradolfer, David
通讯作者：
Kradolfer, David