Conformational changes of the histidine ATP-binding cassette transporter studied by double electron-electron resonance spectroscopy.
Conformational changes of the histidine ATP-binding cassette transporter studied by double electron-electron resonance spectroscopy.
复制标题
双电子-电子共振光谱研究组氨酸 ATP 结合盒转运蛋白的构象变化
DOI：
10.1016/j.bbamem.2014.02.010
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Steinhoff H-J
中科院分区：
文献类型：
--
作者：
Sippach M;Weidlich D;Klose D;Abé C;Klare J;Schneider E;Steinhoff H-J
通过定点自旋标记和双电子-电子共振光谱研究了来自鼠伤寒沙门氏菌的组氨酸 ABC 转运蛋白 HisQMP2 的构象动力学，该转运蛋白来自鼠伤寒沙门氏菌，在无核苷酸、ATP 结合和水解后状态下重组为脂质体。结果表明，完整转运蛋白中 HisP2 Q 环之间测量的二聚体间距离与在水解循环的所有三种状态下为麦芽糖转运蛋白测定的距离相似。只有在配体 HisJ 存在的情况下，才会实现核苷酸结合位点的闭合构象，从而揭示底物存在的跨膜通讯。通过位置 86 和 96 或 104 和 197 之间的旋间距离分布差异检测，可以区分 HisQMP2 的周质部分的两种构象状态。观察到的构象变化与核苷酸结合域 HisP2 的开放、半开放和闭合构象相关。我们的结果与HisQM的跨膜螺旋4和4'在HisP2的封闭到半开放转变过程中的重排是一致的，这是由水解时发生的耦合螺旋3a和3b的重新定向驱动的。
The conformational dynamics of the histidine ABC transporter HisQMP2fromSalmonella entericaserovar Typhimurium, reconstituted into liposomes, is studied by site-directed spin labeling and double electron–electron resonance spectroscopy in the absence of nucleotides, in the ATP-bound, and in the post-hydrolysis state. The results show that the inter-dimer distances as measured between the Q-loops of HisP2in the intact transporter resemble those determined for the maltose transporter in all three states of the hydrolysis cycle. Only in the presence of liganded HisJ the closed conformation of the nucleotide binding sites is achieved revealing the transmembrane communication of the presence of substrate. Two conformational states can be distinguished for the periplasmic moiety of HisQMP2as detected by differences in distributions of interspin distances between positions 86 and 96 or 104 and 197. The observed conformational changes are correlated to proposed open, semi-open and closed conformations of the nucleotide binding domains HisP2. Our results are in line with a rearrangement of transmembrane helices 4 and 4′ of HisQM during the closed to the semi-open transition of HisP2driven by the reorientation of the coupled helices 3a and 3b to occur upon hydrolysis.
登录
查看更多内容
影响因子：
6.8
作者：
Chen J
通讯作者：
Chen J
DOI：
10.2210/pdb1laf/pdb
发表时间：
1995-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Byung-Ha Oh;G. Ames;Sung-HouK Kim
通讯作者：
Byung-Ha Oh;G. Ames;Sung-HouK Kim
影响因子：
9.7
作者：
J. Klare;H. Steinhoff
通讯作者：
H. Steinhoff
影响因子：
3.3
作者：
Polyhach, Yevhen;Bordignon, Enrica;Jeschke, Gunnar
通讯作者：
Jeschke, Gunnar
影响因子：
3.4
作者：
Grote, Mathias;Bordignon, Enrica;Schneider, Erwin
通讯作者：
Schneider, Erwin