Deorphanization and target validation of cross-tick species conserved novel Amblyomma americanum tick saliva protein.
Deorphanization and target validation of cross-tick species conserved novel Amblyomma americanum tick saliva protein.
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DOI：
10.1016/j.ijpara.2012.12.012
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发表时间：
2013-05
影响因子：
4
通讯作者：
Ibelli, Adriana Mercia Guaratini
中科院分区：
文献类型：
--
作者：
Mulenga, Albert;Kim, Tae Kwon;Ibelli, Adriana Mercia Guaratini
关键词：
We previously identified a cross-tick species conserved tick feeding stimuli responsive Amblyomma americanum (Aam) AV422 gene. This study demonstrates that AamAV422 belongs to a novel group of arthropod proteins that is characterized by 14 cysteine amino acid residues: C23-X7/9-C33-X23/24-C58-C8-C67X7-X75-X23-C99-X15-C115-X10-C126X24/25/33-C150C151-X7-C159-X8-X168-X23/24-C192-X9/10-C202 predicted to form seven disulfide bonds. We show that AamAV422 protein is a ubiquitously expressed protein that is injected into the host within the first 24 h of the tick attaching onto the host as revealed by western blotting analyses of recombinant (r)AamAV422, tick saliva and dissected tick organ protein extracts using antibodies to 24 h and 48 h tick saliva proteins (TSPs). Native AamAV422 is apparently involved with mediating tick anti-hemostasis and anti-complement functions in that rAamAV422 delayed plasma clotting time in a dose responsive manner by up to ~160 s, prevented platelet aggregation by up to ~16% and caused ~24% reduction in production of terminal complement complexes. Target validation analysis revealed that rAamAV422 is a potential candidate for a cocktail or multivalent tick vaccine preparation in that RNA interference (RNAi)-mediated silencing of AamAV422 mRNA caused a statistically significant (~44%) reduction in tick engorgement weights, which is proxy for amounts of ingested blood. We speculate that AamAV422 is a potential target antigen for development of the highly desired universal tick vaccine in that consistent with high conservation among ticks, antibodies to 24 h Ixodes scapularis TSPs specifically bound rAamAV422. We discuss data in this study in the context of advancing the biology of tick feeding physiology and discovery of potential target antigens for tick vaccine development.
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影响因子：
6.7
作者：
Dai J;Narasimhan S;Zhang L;Liu L;Wang P;Fikrig E
通讯作者：
Fikrig E
Ir-CPI 是一种来自蓖麻蜱的凝血接触相抑制剂，可抑制血栓形成而不损害止血。
DOI：
10.1084/jem.20091007
发表时间：
2009-10-26
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Decrem Y;Rath G;Blasioli V;Cauchie P;Robert S;Beaufays J;Frère JM;Feron O;Dogné JM;Dessy C;Vanhamme L;Godfroid E
通讯作者：
Godfroid E
影响因子：
2.9
作者：
DAVIE, EW;FUJIKAWA, K;KISIEL, W
通讯作者：
KISIEL, W
影响因子：
5.8
作者：
Ferrè, F;Clote, P
通讯作者：
Clote, P
DOI：
10.4269/ajtmh.2004.71.3.0700268
发表时间：
2004-09-01
影响因子：
3.3
作者：
Ebel, GD;Kramer, LD
通讯作者：
Kramer, LD