Lactobacillus GG and tributyrin supplementation reduce antibiotic-induced intestinal injury.
Lactobacillus GG and tributyrin supplementation reduce antibiotic-induced intestinal injury.
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DOI：
10.1177/0148607113486809
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Ganapathy V
中科院分区：
文献类型：
--
作者：
Cresci G;Nagy LE;Ganapathy V
抗生素治疗会对肠道菌群产生负面影响。乳酸菌GG （LGG）可减轻抗生素相关性腹泻（AAD）症状，但其机制尚不清楚。丁酸盐对肠道健康有益。肠道基因表达的改变发生在缺乏肠道微生物群的情况下。我们假设抗生素引起的肠道微生物群的变化减少了丁酸盐的产生，改变了与肠道屏障完整性和水和电解质吸收有关的基因，从而导致了AAD，同时补充LGG和/或三丁酸甘油酯可以防止这些变化。6 ~ 8周龄的C57BL/6小鼠以周粮喂养，分为±生理盐水、±LGG、±三丁酸甘油酯或两种处理组。小鼠口服±广谱抗生素治疗7 d。记录每日饮水量并测量体重。获得肠组织样本，并通过聚合酶链反应和免疫组织化学分析参与水和电解质吸收、丁酸盐运输和肠道完整性的基因和蛋白质的表达。抗生素降低了肠道内信使RNA （mRNA）表达（丁酸转运体和受体、Na+/H+交换器、Cl - /HCO3 -和水通道）和蛋白质表达（丁酸转运体、Na+/H+交换器和紧密连接蛋白）。添加LGG和/或三丁酸甘油酯使肠道mRNA表达量维持在对照组水平，三丁酸甘油酯使肠道蛋白强度表达维持在对照组水平。广谱抗生素降低小鼠肠道阴离子交换剂、丁酸转运体和受体以及紧密连接蛋白的表达。同时口服补充LGG和/或三丁酸甘油酯可使这些损失最小化。用LGG和/或三丁酸甘油酯优化肠道健康可能为AAD提供预防性治疗。
Antibiotic therapy negatively alters the gut microbiota. Lactobacillus GG (LGG) decreases antibiotic-associated diarrhea (AAD) symptoms, but the mechanisms are unknown. Butyrate has beneficial effects on gut health. Altered intestinal gene expression occurs in the absence of gut microbiota. We hypothesized that antibiotic-induced changes in gut microbiota reduce butyrate production, varying genes involved with gut barrier integrity and water and electrolyte absorption, lending to AAD, and that simultaneous supplementation with LGG and/or tributyrin would prevent these changes. C57BL/6 mice aged 6–8 weeks received a chow diet while divided into 8 treatment groups (± saline, ± LGG, ± tributyrin, or both). Mice received treatments orally for 7 days with ± broad-spectrum antibiotics. Water intake was recorded daily and body weight was measured. Intestine tissue samples were obtained and analyzed for expression of genes and proteins involved with water and electrolyte absorption, butyrate transport, and gut integrity via polymerase chain reaction and immunohistochemistry. Antibiotics decreased messenger RNA (mRNA) expression (butyrate transporter and receptor, Na+/H+ exchanger, Cl−/HCO3−, and a water channel) and protein expression (butyrate transporter, Na+/H+ exchanger, and tight junction proteins) in the intestinal tract. LGG and/or tributyrin supplementation maintained intestinal mRNA expression to that of the control animals, and tributyrin maintained intestinal protein intensity expression to that of control animals. Broad-spectrum antibiotics decrease expression of anion exchangers, butyrate transporter and receptor, and tight junction proteins in mouse intestine. Simultaneous oral supplementation with LGG and/or tributyrin minimizes these losses. Optimizing intestinal health with LGG and/or tributyrin may offer a preventative therapy for AAD.
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影响因子：
64.8
作者：
Brandl, Katharina;Plitas, George;Mihu, Coralia N.;Ubeda, Carles;Jia, Ting;Fleisher, Martin;Schnabl, Bernd;DeMatteo, Ronald P.;Pamer, Eric G.
通讯作者：
Pamer, Eric G.
影响因子：
3.6
作者：
De La Cochetiere, M. F.;Durand, T.;Beaugerie, L.
通讯作者：
Beaugerie, L.
DOI：
10.1016/s0924-8579(00)00293-4
发表时间：
2000-12-01
影响因子：
10.8
作者：
Bergogne-Bérézin, E
通讯作者：
Bergogne-Bérézin, E
影响因子：
5.5
作者：
Mace, Oliver J.;Affleck, Julie;Kellett, George L.
通讯作者：
Kellett, George L.
影响因子：
3
作者：
Egorin, MJ;Yuan, ZM;Eiseman, JL
通讯作者：
Eiseman, JL