Sequence signatures of two public antibody clonotypes that bind SARS-CoV-2 receptor binding domain.
Sequence signatures of two public antibody clonotypes that bind SARS-CoV-2 receptor binding domain.
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DOI：
10.1038/s41467-021-24123-7
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发表时间：
2021-06-21
影响因子：
16.6
通讯作者：
Wu NC
中科院分区：
文献类型：
--
作者：
Tan TJC;Yuan M;Kuzelka K;Padron GC;Beal JR;Chen X;Wang Y;Rivera-Cardona J;Zhu X;Stadtmueller BM;Brooke CB;Wilson IA;Wu NC
自新冠肺炎大流行以来，对SARS-CoV-2的抗体反应有广泛的特征。针对Spike蛋白受体结合域(RBD)的抗体通常由IGHV3-53/3-66编码，带有一个短互补决定区(CDR)H3。重链CDR H1和H2中的种系编码序列基序具有主要功能，但CDR H3中是否存在共同的基序尚不清楚，CDR H3通常对结合特异性至关重要。在这里，我们鉴定了两种公共克隆类型的IGHV3-53/3-66 RBD抗体，其中9个残基为CDR H3，与不同的轻链配对。CDR H3上不同的序列基序存在于两个公共克隆类型中，这两种克隆似乎与差异轻链配对有关。此外，我们还发现Y58F是一种常见的体细胞超突变，它导致IGHV3-53/3-66 RBD抗体与短CDR H3的结合亲和力增加。这些结果促进了对SARS-CoV-2抗体反应的理解。识别SARS-CoV-2刺突蛋白的公共抗体克隆型对于抵御新冠肺炎是重要的。在这里，作者表征了两个公共克隆型的重链互补决定区(CDR)H3S的序列基序及其与轻链同一性的关系。
Since the COVID-19 pandemic onset, the antibody response to SARS-CoV-2 has been extensively characterized. Antibodies to the receptor binding domain (RBD) on the spike protein are frequently encoded by IGHV3-53/3-66 with a short complementarity-determining region (CDR) H3. Germline-encoded sequence motifs in heavy chain CDRs H1 and H2 have a major function, but whether any common motifs are present in CDR H3, which is often critical for binding specificity, is not clear. Here, we identify two public clonotypes of IGHV3-53/3-66 RBD antibodies with a 9-residue CDR H3 that pair with different light chains. Distinct sequence motifs on CDR H3 are present in the two public clonotypes that seem to be related to differential light chain pairing. Additionally, we show that Y58F is a common somatic hypermutation that results in increased binding affinity of IGHV3-53/3-66 RBD antibodies with a short CDR H3. These results advance understanding of the antibody response to SARS-CoV-2. Public antibody clonotypes that recognize SARS-CoV-2 spike protein are important for protection against COVID-19. Here, the authors characterize sequence motifs in the heavy chain complementarity-determining region (CDR) H3s of two public clonotypes and their association with light chain identity.
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影响因子：
64.8
作者：
Barnes CO;Jette CA;Abernathy ME;Dam KA;Esswein SR;Gristick HB;Malyutin AG;Sharaf NG;Huey-Tubman KE;Lee YE;Robbiani DF;Nussenzweig MC;West AP Jr;Bjorkman PJ
通讯作者：
Bjorkman PJ
影响因子：
64.8
作者：
Gaebler C;Wang Z;Lorenzi JCC;Muecksch F;Finkin S;Tokuyama M;Cho A;Jankovic M;Schaefer-Babajew D;Oliveira TY;Cipolla M;Viant C;Barnes CO;Bram Y;Breton G;Hägglöf T;Mendoza P;Hurley A;Turroja M;Gordon K;Millard KG;Ramos V;Schmidt F;Weisblum Y;Jha D;Tankelevich M;Martinez-Delgado G;Yee J;Patel R;Dizon J;Unson-O'Brien C;Shimeliovich I;Robbiani DF;Zhao Z;Gazumyan A;Schwartz RE;Hatziioannou T;Bjorkman PJ;Mehandru S;Bieniasz PD;Caskey M;Nussenzweig MC
通讯作者：
Nussenzweig MC
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
15
作者：
Chelli, R;Gervasio, FL;Schettino, V
通讯作者：
Schettino, V
影响因子：
64.5
作者：
Du, Shuo;Cao, Yunlong;Qin, Chuan
通讯作者：
Qin, Chuan