Improved gene transfer by direct plasmid injection associated with regeneration in mouse skeletal muscle

Improved gene transfer by direct plasmid injection associated with regeneration in mouse skeletal muscle
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通过直接质粒注射改善与小鼠骨骼肌再生相关的基因转移

DOI:
10.1016/0014-5793(93)80508-r
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发表时间:
1993
期刊:
影响因子:
3.5
通讯作者:
D. Wells
D. Wells
中科院分区:
生物学3区
文献类型:
--
作者:
D. Wells

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Gene transfer into skeletal muscle via simple plasmid injection in vivo has many potential uses but these are severely constrained by the low efficiency of this technique. Muscle regeneration, induced by the myotoxic local anaesthetic bupivacaine, significantly increased gene expression following plasmid injection 3–7 days after bupivacaine treatment. Much of this effect can be attributed to uptake and expression of the plasmid by a greater number of muscle fibres, up to 9% of the mouse tibialis anterior muscle. A similar significant increase in expression was observed in the naturally regenerating muscle of the dystrophicmdxmouse when compared to the control C57B1/10 strain.
DOI: 10.1161/01.cir.82.6.2217
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发表时间: 1992-02-01
期刊: HUMAN GENE THERAPY
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DOI: --
发表时间: 1993
期刊: Laboratory investigation; a journal of technical methods and pathology
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Gal,D;Weir,L;Leclerc,G;Pickering,JG;Hogan,J;Isner,JM
通讯作者: Isner,JM