Visualizing SNVs to quantify allele-specific expression in single cells.
Visualizing SNVs to quantify allele-specific expression in single cells.
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DOI:
10.1038/nmeth.2589
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发表时间:
2013-09
期刊:
影响因子:
48
通讯作者:
Raj, Arjun
中科院分区:
文献类型:
--
作者:
Levesque, Marshall J.;Ginart, Paul;Wei, Yichen;Raj, Arjun
We present a high efficiency fluorescence in situ hybridization method for detecting single nucleotide variants (SNVs) on individual RNA transcripts, both exonic and intronic. We used this method to quantify allelic expression at the population and single cell level, and also to distinguish maternal from paternal chromosomes in single cells.
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