Ion-selective optodes measure extracellular potassium flux in excitable cells.
Ion-selective optodes measure extracellular potassium flux in excitable cells.
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DOI：
10.1002/marc.200900297
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发表时间：
2010-01-18
影响因子：
4.6
通讯作者：
Clark, Heather A.
中科院分区：
文献类型：
--
作者：
Harjes, Daniel I.;Dubach, J. Matthew;Rosenzweig, Anthony;Das, Saumya;Clark, Heather A.
光电二极管用于离子浓度和通量的检测已有数年。然而，它们在生物医学应用中的用途尚未得到充分探索。本研究探讨了光电极作为一种潜在的传感器平台，用于监测细胞离子通量，并在药物筛选和毒理学领域产生影响。开发了一个原型系统来定量测量单层心肌细胞的细胞外钾通量。产生光极并将其固定在玻璃盖玻片上用于荧光成像。该系统检测钾（K+）离子通量在心脏动作电位的复极化阶段，并进一步检测到一个已知的K+通道抑制剂的存在下，通过光学监测局部K+离子浓度的心肌细胞单层的场刺激过程中的通量的幅度的减少。
Optodes have been used for detection of ionic concentrations and fluxes for several years. However, their uses in biomedical applications have not yet been fully explored. This study investigates optodes as a potential sensor platform for monitoring cellular ion flux with attendant implications in the field of drug screening and toxicology. A prototype system was developed to quantitatively measure extracellular potassium flux from a monolayer of cardiomyocytes. Optodes were created and immobilized on a glass coverslip for fluorescent imaging. The system detected potassium (K+) ion flux during the repolarization phase of the cardiac action potential and further detected a decrease in the magnitude of the flux in the presence of a known K+ channel inhibitor by optically monitoring local K+ ion concentrations during field stimulation of the cardiomyocyte monolayer.
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影响因子：
7.4
作者：
Clark, HA;Kopelman, R;Philbert, MA
通讯作者：
Philbert, MA
影响因子：
20.1
作者：
Morissette, Michael R.;Cook, Stuart A.;Rosenzweig, Anthony
通讯作者：
Rosenzweig, Anthony
影响因子：
64.8
作者：
BENNETT, PB;YAZAWA, K;GEORGE, AL
通讯作者：
GEORGE, AL
影响因子：
10.8
作者：
Dubach, J. Matthew;Harjes, Daniel I.;Clark, Heather A.
通讯作者：
Clark, Heather A.
影响因子：
7.4
作者：
Clark, HA;Hoyer, M;Kopelman, R
通讯作者：
Kopelman, R