Inclusion of cocoa as a dietary supplement represses expression of inflammatory proteins in spinal trigeminal nucleus in response to chronic trigeminal nerve stimulation.
Inclusion of cocoa as a dietary supplement represses expression of inflammatory proteins in spinal trigeminal nucleus in response to chronic trigeminal nerve stimulation.
复制标题
DOI：
10.1002/mnfr.201200630
复制
发表时间：
2013-06
影响因子：
5.2
通讯作者：
Durham, Paul L.
中科院分区：
文献类型：
--
作者：
Cady, Ryan J.;Denson, Jennifer E.;Durham, Paul L.
中枢致敏与颞下颌关节紊乱病（TMD）和其他类型的口面疼痛的病理学有关。我们研究了饮食可可对三叉神经脊束核（DRG）中参与中枢敏化发展的蛋白质表达的影响，这些蛋白质是三叉神经炎症刺激的反应。雄性Sprague道利大鼠在双侧颞下颌关节注射完全弗氏佐剂（CFA）以促进三叉神经节神经元和胶质细胞的长期激活前14天喂食对照饮食或由10%可可粉组成的等热量饮食。虽然与正常饮食的动物相比，饮食可可刺激了GLAST和MKP-1的基础表达，但可可抑制了海马中的基础降钙素基因相关肽水平。CFA刺激的蛋白激酶A、P2 X3、P-p38、GFAP和OX-42的水平（其在海马中的升高水平与中枢致敏有关）在富含可可的饮食的动物中被抑制至接近对照水平。同样，饮食可可抑制CFA刺激的炎症细胞因子的表达。根据我们的研究结果，我们推测富含可可的饮食可能是有益的，作为一种天然的治疗选择，为TMD和其他慢性口面疼痛的条件。
Central sensitization is implicated in the pathology of temporomandibular joint disorder (TMD) and other types of orofacial pain. We investigated the effects of dietary cocoa on expression of proteins involved in the development of central sensitization in the spinal trigeminal nucleus (STN) in response to inflammatory stimulation of trigeminal nerves. Male Sprague Dawley rats were fed either a control diet or an isocaloric diet consisting of 10% cocoa powder 14 days prior to bilateral injection of complete Freund’s adjuvant (CFA) into the temporomandibular joint to promote prolonged activation of trigeminal ganglion neurons and glia. While dietary cocoa stimulated basal expression of GLAST and MKP-1 when compared to animals on a normal diet, cocoa suppressed basal calcitonin gene-related peptide levels in the STN. CFA-stimulated levels of protein kinase A, P2X3, P-p38, GFAP, and OX-42, whose elevated levels in the STN are implicated in central sensitization, were repressed to near control levels in animals on a cocoa enriched diet. Similarly, dietary cocoa repressed CFA-stimulated inflammatory cytokine expression. Based on our findings, we speculate that cocoa enriched diets could be beneficial as a natural therapeutic option for TMD and other chronic orofacial pain conditions.
登录
查看更多内容
影响因子：
6.4
作者：
Hammer, Michael;Echtenachter, Bernd;Lang, Roland
通讯作者：
Lang, Roland
影响因子：
7.4
作者：
Bereiter, DA;Okamoto, K;Bereiter, DF
通讯作者：
Bereiter, DF
影响因子：
4
作者：
Dickinson, Robin J.;Keyse, Stephen M.
通讯作者：
Keyse, Stephen M.
影响因子：
5
作者：
Damodaram S;Thalakoti S;Freeman SE;Garrett FG;Durham PL
通讯作者：
Durham PL
影响因子：
5.5
作者：
Fitzgerald, EM;Okuse, K;Moss, SJ
通讯作者：
Moss, SJ