Changes in the Endometrial Transcriptome During the Bovine Estrous Cycle: Effect of Low Circulating Progesterone and Consequences for Conceptus Elongation1
Changes in the Endometrial Transcriptome During the Bovine Estrous Cycle: Effect of Low Circulating Progesterone and Consequences for Conceptus Elongation1
复制标题
牛发情周期期间子宫内膜转录组的变化：低循环黄体酮的影响及其对概念延长的影响1
作者：
N. Forde;M. Beltman;G. Duffy;P. Duffy;J. Mehta;P. O'Gaora;J. Roche;P. Lonergan;M. Crowe
在牛中，在受孕后立即升高的循环孕酮(P4)浓度与晚期胚胎发育有关，而低P4可能是奶牛低妊娠率的原因之一。本研究的目的是：1)描述发情周期中牛子宫内膜的转录变化，2)确定P4升高对这些变化的影响，3)确定低P4是否改变了这些基因的表达，以及4)评估低P4对受孕发育的影响。在发情周期的黄体早期(第5天和第7天)，子宫内膜基因表达的差异相对较小，但对黄体期较远阶段的子宫内膜(第7天和第13天)的比较发现，转录发生了巨大的变化，这种变化显著地被外源补充P4所改变。低循环P4的诱导改变了基因表达的正常时间变化，这些变化与LE和GE下调PGR的延迟相一致。低P4诱导的子宫内膜基因表达变化与胚胎移植后第7天子宫对胚胎发育的支持能力降低有关。总之，本研究提供了明确的证据，表明动情后最初几天，周期小母牛子宫内膜转录组的时间变化对循环中P4浓度的变化很敏感。在低P4条件下，观察到支持妊娠延长的能力降低的次优子宫环境。
In cattle, elevated concentrations of circulating progesterone (P4) in the immediate postconception period are associated with advanced conceptus development, while low P4 is implicated as a causative factor in low pregnancy rates observed in dairy cows. This study aimed to: 1) describe the transcriptional changes that occur in the bovine endometrium during the estrous cycle, 2) determine how elevated P4 affects these changes, 3) identify if low P4 alters the expression of these genes, and 4) assess the impact that low P4 has on conceptus development. Relatively few differences occurred in endometrial gene expression during the early luteal phase of the estrous cycle (Day 5 vs. 7), but comparison of endometria from more distant stages of the luteal phase (Day 7 vs. 13) revealed large transcriptional changes, which were significantly altered by exogenous supplementation of P4. Induction of low circulating P4 altered the normal temporal changes in gene expression, and these changes were coordinate with a delay in the down-regulation of the PGR from the LE and GE. Altered endometrial gene expression induced by low P4 was associated with a reduced capacity of the uterus to support conceptus development after embryo transfer on Day 7. In conclusion, the present study provides clear evidence that the temporal changes in the transcriptome of the endometrium of cyclic heifers are sensitive to circulating P4 concentrations in the first few days after estrus. Under low P4 conditions, a suboptimal uterine environment with reduced ability to support conceptus elongation is observed.
影响因子：
5.8
作者：
Lutz, Justin D.;Dixit, Vaishali;Yeung, Catherine K.;Dickmann, Leslie J.;Zelter, Alex;Thatcher, Jayne E.;Nelson, Wendel L.;Isoherranen, Nina
通讯作者：
Isoherranen, Nina
影响因子：
3.6
作者：
G. A. Surveyor;Amy K. Wilson;D. Brigstock
通讯作者：
G. A. Surveyor;Amy K. Wilson;D. Brigstock
结缔组织生长因子在人体子宫组织中的定位。
影响因子：
4
作者：
Uzumcu,M;Homsi,MF;Ball,DK;Coskun,S;Jaroudi,K;Hollanders,JM;Brigstock,DR
通讯作者：
Brigstock,DR