Trichinella spiralis: antigenic epitopes from the stichocytes detected in the hypertrophic nuclei and cytoplasm of the parasitized muscle fibre (nurse cell) of the host
Trichinella spiralis: antigenic epitopes from the stichocytes detected in the hypertrophic nuclei and cytoplasm of the parasitized muscle fibre (nurse cell) of the host
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旋毛虫：在宿主寄生的肌纤维（护士细胞）的肥大细胞核和细胞质中检测到的来自线形细胞的抗原表位
DOI：
10.1017/s003118200006042x
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发表时间：
1991
期刊：
影响因子：
2.4
通讯作者：
M. Yeung
中科院分区：
文献类型：
--
作者：
D.;L.;Lee;R.;KO C.;YI X.Y.;M. Yeung
总结针对旋毛虫幼虫排泄物/分泌物（E/S）中存在的抗原产生的单克隆抗体、针对E/S产生的多克隆抗体以及来自感染旋毛虫的兔和猪的抗血清。将旋毛虫与免疫细胞化学技术结合使用，以检测感染旋毛虫的小鼠肌肉切片中的抗原。15或30天。抗体识别的表位在stichocytes，在角质层的表面上，在食道的内腔和在肠腔的包囊幼虫。单克隆抗体7C2C5和1H7和多克隆抗体识别幼虫所占据的腔中、保育细胞的细胞质中和保育细胞的肥大细胞核中的表位，但不识别保育细胞的较小细胞核中、囊壁中或周围肌肉中的材料。单克隆抗体3B2E6和1D11G8B2能识别幼虫表皮细胞内和表皮表面的抗原决定簇，但对滋养细胞的胞质和胞核均呈阴性反应。猪鞭虫多克隆抗体识别包囊猪鞭虫肌肉和皮下组织中的抗原表位。而对滋养细胞和滋养细胞核内的物质检测结果均为阴性。的可能性，在细胞质和细胞核的护士细胞中检测到的抗原是由线虫的stichocytes，它是控制基因的改变肌纤维，直接或间接，进行了讨论。
SUMMARY Monoclonal antibodies raised against antigens present in the excretions/secretions (E/S) of larval Trichinella spiralis, polyclonal antibodies raised against E/S and antisera from rabbits and pigs infected with T. spiralis were used in conjunction with immunocytochemical techniques to detect antigens in sections of muscle from mice that had been infected with T. spiralis for 15 or 30 days. The antibodies recognized epitopes in the stichocytes, on the surface of the cuticle, in the lumen of the oesophagus and in the lumen of the intestine of encysted larvae. Monoclonal antibodies 7C2C5 and 1H7 and the polyclonal antibodies recognized epitopes in the cavity occupied by the larva, in the cytoplasm of the nurse cell, and in the hypertrophic nuclei of the nurse cell, but did not recognize material in the smaller nuclei of the nurse cell, in the cyst wall or in the surrounding muscle. Monoclonals 3B2E6 and 1D11G8B2, which recognized epitopes in the stichocytes and on the surface of the cuticle of the larvae, gave negative results with the cytoplasm and nuclei of the nurse cell. A polyclonal antibody raised against Trichuris suis recognized epitopes in the muscle and hypodermis of encysted T. spiralis but gave negative results with material in the nurse cell and nurse cell nuclei. The possibility that the antigen detected in the cytoplasm and nuclei of the nurse cell is produced by the stichocytes of the nematode and that it is controlling genes of the altered muscle fibre, either directly or indirectly, is discussed.
来自旋毛虫的抗原可在小鼠中诱导保护性反应。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Silberstein,DS;Despommier,DD
通讯作者：
Despommier,DD
影响因子：
2.1
作者：
DESPOMMIER, DD;GOLD, AM;SILBERSTEIN, D
通讯作者：
SILBERSTEIN, D