Insights Gained and Future Outlook From scRNAseq Studies in Autoimmune Rheumatic Diseases.
Insights Gained and Future Outlook From scRNAseq Studies in Autoimmune Rheumatic Diseases.
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DOI：
10.3389/fimmu.2022.849050
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发表时间：
2022
影响因子：
7.3
通讯作者：
Zou L
中科院分区：
文献类型：
--
作者：
Zheng Z;Chang L;Li J;Wu Y;Chen G;Zou L
自身免疫性风湿性疾病作为最常见的疾病之一对公共卫生具有重大影响，并且这些疾病中的许多涉及局部和全身表现，对患者健康和生活质量具有严重后果。然而，这些疾病中的许多疾病的治疗选择对于相当一部分患者仍然不足，并且开发新疗法的进展缓慢。这种进展的缺乏在很大程度上可归因于对驱动发病机制的复杂机制的理解不足。最近，单细胞RNA测序（scRNAseq）的出现为询问风湿性疾病提供了一个强大的新工具，有可能评估风湿性疾病的生物异质性和个体细胞功能。在这篇综述中，我们讨论了从目前人类风湿性疾病的scRNAseq询问中获得的主要见解。我们强调了新的细胞群和发现的关键分子特征，并提出了一些可能对该领域感兴趣的后续研究的假设。我们还提供了一个展望到两个新兴的单细胞技术（剧目测序和空间转录组学），尚未被用于风湿性疾病领域，但它提供了巨大的潜力，扩大我们的理解免疫和基质细胞的行为。我们希望scRNAseq可以作为产生和询问关于自身反应性淋巴细胞和组织浸润模式的新假设的源泉，并帮助发现治疗开发的新途径。
Autoimmune rheumatic diseases have a major impact on public health as one of the most common morbidities, and many of these disorders involve both local and systemic manifestations with severe consequences for patient health and quality of life. However, treatment options for many of these diseases remain inadequate for a substantial portion of patients, and progress in developing novel therapeutics has been slow. This lack of progress can be largely attributed to an insufficient understanding of the complex mechanisms driving pathogenesis. Recently, the emergence of single-cell RNA sequencing (scRNAseq) has offered a powerful new tool for interrogating rheumatic diseases, with the potential to assess biological heterogeneity and individual cell function in rheumatic diseases. In this review, we discuss the major insights gained from current scRNAseq interrogations of human rheumatic diseases. We highlight novel cell populations and key molecular signatures uncovered, and also raise a number of hypotheses for follow-up study that may be of interest to the field. We also provide an outlook into two emerging single-cell technologies (repertoire sequencing and spatial transcriptomics) that have yet to be utilized in the field of rheumatic diseases, but which offer immense potential in expanding our understanding of immune and stromal cell behavior. We hope that scRNAseq may serve as a wellspring for the generation and interrogation of novel hypotheses regarding autoreactive lymphocytes and tissue infiltration patterns, and help uncover novel avenues for therapeutic development.
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影响因子：
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作者：
Derksen VFAM;Huizinga TWJ;van der Woude D
通讯作者：
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